黄芩苷对创伤性脑损伤模型大鼠的治疗作用及Akt/Nrf2信号通路的影响

目的 探讨黄芩苷对创伤性脑损伤（TBI）模型大鼠的治疗作用及蛋白激酶B(Akt)/核因子E2相关因子2(Nrf2)信号通路的影响。方法 建立TBI模型大鼠，按照随机数字表法分成模型组、阳性对照组（单唾液酸四己糖神经节苷脂钠注射液14 mg/kg）及黄芩苷低、中、高剂量组（50、100、200 mg/kg），每组12只；另取12只大鼠设为假手术组。检测大鼠神经功能缺损评分（mNSS）、脑组织含水量、脑组织Akt、Nrf2 mRNA和蛋白表达水平，并观察大鼠脑组织病理结构。结果 假手术组大鼠脑组织细胞结构完整、排列整齐，未见出血及水肿；模型组大鼠脑组织细胞排列松散，且部分细胞点状坏死，大量炎性细胞浸润，出血和水肿明显；黄芩苷低、中、高剂量组大鼠脑组织出血、水肿面积和点状坏死细胞数量逐渐减少；阳性对照组和黄芩苷高剂量组效果相近。与假手术组比较，模型组大鼠mNSS评分[（8.43±1.72）分比（0.37±0.04）分，P<0.05]、脑组织含水量[（82.93±2.19）%比（68.72±1.36）%,P<0.05]升高，脑组织Akt、Nrf2 mRNA[Akt mRNA:(0....

Therapeutic effect of baicalin on traumatic brain injury in rats and the effect of Akt/Nrf2 signal pathway

Objective: To investigate the therapeutic effect of baicalin on traumatic brain injury (TBI) rats and the effect of protein kinase B (Akt) / nuclear factor E2 related factor 2 (Nrf2) signal pathway. Methods: TBI rat model was established and randomly divided into model group, positive control group (monosialotetrahexosylganglioside sodium injection 14 mg/kg), baicalin low dose group (50 mg/kg), baicalin medium dose group (100 mg/kg) and baicalin high dose group (200 mg/kg), with 12 rats in each group; Another 12 rats were set as sham operation group. The modified neurological severity score (mNSS), brain water content, brain tissue Akt, Nrf2 messenger RNA (mRNA) and protein expression levels of rats were measured, and the pathological structure of brain tissue in rats was observed. Results: In the sham operation group, the structure of brain tissue cells in rats was complete and orderly, and there was no bleeding and edema; in the model group, the arrangement of brain tissue cells in rats was loose, and some cells were punctate necrosis, a large number of inflammatory cell infiltration, bleeding and edema were obvious; the area of cerebral hemorrhage, edema and the number of punctate necrotic cells decreased gradually of rats in baicalin low, medium and high dose groups; the effects of positive control group and baicalin high dose group were similar. Compared with the sham operation group, the mNSS score and brain water content of rats in the model group were increased, and the expression levels of Akt, Nrf2 mRNA and protein in the brain tissue were decreased (P<0.05); compared with the model group, the mNSS score and brain water content of rats in the positive control group and baicalin low, medium and high dose groups were decreased, and the expression levels of Akt, Nrf2 mRNA and protein in the brain tissue were increased, there was a dose-dependent relationship between baicalin dose groups (P<0.05); there was no significant difference in the above indexes between the positive control group and the baicalin high dose group (P>0.05). Conclusion: Baicalin can effectively treat brain nerve injury induced by TBI in rats and improve brain edema, its mechanism may be related to baicalin activating Akt/Nrf2 signal pathway and promoting the expression of Akt and Nrf2 mRNA and protein.