皮肤型红斑狼疮皮损中鳞状细胞癌抗原1的表达和意义

目的:研究鳞状细胞癌抗原1(squamous cell carcinoma antigen-1,SSCA1)在皮肤型红斑狼疮(cutaneous lupus erythematosus,CLE)皮损中的表达和意义?方法:培养原代角质形成细胞,提取蛋白样品采用固相pH梯度双向凝胶电泳进行分离,应用ImageMaster 2D Platinum 5.0软件对图像进行匹配分析,选取差异表达个蛋白质点,经基质辅助激光解吸附飞行时间质谱进行质谱鉴定?并通过免疫印迹验证其表达水平?结果:体外培养角质形成细胞成功,获得重复性较好的双向电泳图谱,和正常皮肤相比,SCCA1在CLE皮损角质形成细胞中表达升高,免疫印迹验证与双向电泳结果一致?结论:SCCA1在CLE皮损角质形成细胞中表达升高,可能与CLE的发生发展有关,但SCCA1表达升高的具体机制仍需进一步研究?

The expression and significance of squamous cell carcinoma antigen-1 in cutaneous lupus erythematosus

Objective: To study the expression and significance of squamous cell carcinoma antigen-1 (SCCA1) in keratinocytes from the lesion of cutaneous lupus erythematosus. Methods: The keratinocytes were isolated from the human skin and cultured in serum-free media. The total proteins of keratinocytes were separated by 2-dimensional gel electrophoresis. The differential proteins were analyzed using ImageMaster 2D analysis software after staining and identified by MALDI-TOF-MS. The expressional levels of the proteins were further confirmed by Western blot analysis. Results: We successfully in vitro cultured human skin primary keratinocytes and established 2-dimensional electrophoresis profiles with high resolution and reproducibility. The SCCA1 protein was up-regulated in the keratinocytes from lesion of CLE compared to the normal group,and the result from both proteomics approach agreed with that of Western blot analysis. Conclusion: The SCCA1 protein was up-regulated in the keratinocytes from lesion of CLE,which may be related to the development of CLE. However,the pathogenesis of over expression of SCCA1 need further study.