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| 恶性淋巴瘤中p16基因的缺失及甲基化 | |
| 引用本文： | 药锦娟,许良中,施宗高,朱伟萍,张泰明,金爱萍,李小妹. 恶性淋巴瘤中p16基因的缺失及甲基化[J]. 中国癌症杂志, 2000, 10(5): 394-396 |
| 作者姓名： | 药锦娟 许良中 施宗高 朱伟萍 张泰明 金爱萍 李小妹 |
| 作者单位： | 复旦大学医学院附属肿瘤医院病理科,上海,200032 |
| 基金项目： | 国家自然科学基金(编号:39870295) |
| 摘 要： | 目的：研究恶性淋巴瘤中ｐ１６基因缺失、甲基化发生的频率及其在淋巴瘤发生、发展中的作用，并研究其与淋巴瘤恶性度的关系。方法：收集７８例新鲜淋巴瘤标本及９例反应性增生的标本，用聚合酶链反应（ＰＣＲ）扩增ｐ１６基因第１、第２外显子，检测等位基因纯合性缺失，用限制性内切酶－ＰＣＲ方法检测ｐ１６基因甲基化。结果：ｐ１６基因在恶性淋巴瘤中的缺失率为１１．５％，甲基化率为２６．９％；９例反应性增生未见ｐ１６基因 |
| 关 键 词： | 淋巴肉瘤 基因 p16 缺失 甲基化 发病机制 |
| 文章编号： | 1007-3639(2000)05-0394-03 |
| 修稿时间： | 2000-04-10 |
Homozygous deletion and methylation of p16 gene in malignant lymphoma |
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| YAO Jin juan,XU Liang zhong,SHI Zong gao,et al. Homozygous deletion and methylation of p16 gene in malignant lymphoma[J]. China Oncology, 2000, 10(5): 394-396 | |
| Authors: | YAO Jin juan XU Liang zhong SHI Zong gao et al |
| Abstract: | Purpose:To investigate the frequency of p16 gene inactivation in malignant lymphoma and to evaluate its clinicopathological correlation. Methods:Seventy eight patients with newly diagnosed lymphoma were studied, PCR technique was used to detect homozygous deletion of p16 gene, restriction enzyme PCR technique was used to detect the 5' CpG island methylation. Results:p16 gene homozygous deletion was found in 9 and methylation in 21 patients. Conclusions:Hypermethylaiton is the main way of p16 gene inactivation in malignant lymphoma. The inactivation of p16 gene may play a key role in the pathogenesis of malignant lymphoma. |
| Keywords: | lymphoma gene p16 gene deletion methylation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |