不同形态梅花鹿鹿茸的化学成分对比研究

目的研究不同形态梅花鹿鹿茸化学成分的差异。方法采用紫外分光光度计、杜马斯定氮仪、全自动氨基酸分析仪、超高效液相色谱仪、高效液相色谱仪、气相色谱仪、电感耦合等离子质谱仪、原子荧光光度计等测定并比较了梅花鹿毛桃茸、二杠茸、三岔茸中多糖、粗蛋白、氨基酸、胶原蛋白、脂肪酸、矿质元素、生物胺、核苷、硫酸软骨素9类化学成分的含量差异,用主成分分析(PCA)法综合评价不同形态梅花鹿鹿茸的质量。结果多糖、粗蛋白、氨基酸、胶原蛋白、脂肪酸、矿质元素、生物胺、核苷、硫酸软骨素在毛桃茸中质量分数分别为8.40 mg/g、44.82%、42.24%、23.23%、6 234.69 mg/kg、145.69 mg/g、55.12 mg/kg、2 271.87 mg/kg、0.74 mg/g;在二杠茸中质量分数分别为8.14 mg/g、52.12%、48.57%、21.50%、8 684.27 mg/kg、126.40 mg/g、76.14 mg/kg、3 438.37 mg/kg、1.94 mg/g;在三岔茸中质量分数分别为8.64 mg/g、51.86%、45.49%、22.31%、9 100.78 mg/kg、138.36 mg/g、70.75 mg/kg、2 507.82 mg/kg、1.84 mg/g。结论不同形态梅花鹿鹿茸的化学成分有差异,PCA结果显示,二杠茸质量最好,三岔茸次之,毛桃茸质量最差。该研究为不同形态鹿茸质量评价及分等、分级标准提供参考。

Comparative study on chemical constituents of Cervi Cornu Pantotrichum in different forms of Cervus nipport

Objective In order to study the differences in chemical constituents of Cervi Cornu Pantotrichum in different forms of Cervus nipport. Methods The content of polysaccharides, crude protein, amino acids, collagen, fatty acids, mineral elements, biogenic amines, nucleosides, and chondroitin sulfate composition of Cervi Cornu Pantotrichum in different forms of C. nipport was determined and compared by using UV spectrophotometer, Dumas azotometer, automatic amino acid analyzer, UPLC, HPLC, gas chromatograph, inductively coupled plasma mass spectrometer and atomic fluorescence photometer. Principal component analysis was used to evaluate the quality of Cervi Cornu Pantotrichum. Results The content of polysaccharide, crude protein, amino acid, collagen, fatty acid, mineral element, biogenic amine, nucleoside and chondroitin sulfate in one-branched velvet antler was 8.40 mg/g, 44.82%, 42.24%, 23.23%, 6 234.69 mg/kg, 145.69 mg/g, 55.12 mg/kg, 2 271.87 mg/kg, and 0.74 mg/g, respectively; The content in two-branched velvet antler was 8.14 mg/g, 52.12%, 48.57%, 21.50%, 8 684.27 mg/kg, 126.40 mg/g, 76.14 mg/kg, 3 438.37 mg/kg, and 1.94 mg/g, respectively; The content in three-branched velvet antler was 8.64 mg/g, 51.86%, 45.49%, 22.31%, 9 100.78 mg/kg, 138.36 mg/g, 70.75 mg/kg, 2 507.82 mg/kg, and 1.84 mg/g, respectively. Conclusion The chemical composition of Cervi Cornu Pantotrichum in different forms of C. nipport was different. The results of principal component analysis showed that the quality of the two-branched velvet antler was the best, the three-branched velvet antler was the second, the one-branched velvet antler was the worst. This study provides a reference for the quality evaluation and grading standards of different forms of Cervi Cornu Pantotrichum.