Identification of Curcuma plants and curcumin content level by DNA polymorphisms in the trnS-trnfM intergenic spacer in chloroplast DNA |
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| Authors: | Motoyasu Minami Kaori Nishio Yui Ajioka Hiroaki Kyushima Katsuki Shigeki Kaneo Kinjo Kazuno Yamada Masashi Nagai Kiyoshi Satoh Yozo Sakurai |
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| Affiliation: | (1) Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai Aichi, 487-8501, Japan;(2) Tanegashima Division, Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation, 17007-2 Noma, Nakatane, Kumage-gun Kagoshima, 891-3604, Japan;(3) Laboratory of Root and Tuber Crop, Horticultural Branch, Okinawa Agricultural Experiment Station, 76 Kanekadan, Gushikawa Okinawa, 904-2241, Japan;(4) R&;D Division, Kinjirushi Co., Ltd, 2-61 Yahata-hontoi, Nakagawa-ku Nagoya, Aichi, 454-8526, Japan;(5) JICA, 1–211 Kitamatsuyama, Seto Aichi, 489-0911, Japan |
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| Abstract: | ![]()
With the goal of developing an accurate plant identification method, molecular analysis based on polymorphisms of the nucleotide sequence of chloroplast DNA (cpDNA) was performed in order to distinguish four Curcuma species: C. longa, C. aromatica, C. zedoaria, and C. xanthorrhiza. Nineteen regions of cpDNA were amplified successfully via polymerase chain reaction (PCR) using total DNA of all Curcuma plants. Using the intergenic spacer between trnS and trnfM (trnSfM), all four Curcuma plant species were correctly identified. In addition, the number of AT repeats in the trnSfM region was predictive of the curcumin content in the rhizome of C. longa. |
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| Keywords: | Chloroplast DNA C. aromatica C. longa C. xanthorrhiza C. zedoaria |
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