| 双黄连颗粒中绿原酸含量的HPLC测定 |
| |
| 引用本文： | 张洪涛,蔡俊安,郭鑫慧. 双黄连颗粒中绿原酸含量的HPLC测定[J]. 中国中医药信息杂志, 2010, 17(4): 43-44 |
| |
| 作者姓名： | 张洪涛 蔡俊安 郭鑫慧 |
| |
| 作者单位： | 河南百年康鑫药业有限公司,河南,郸城,477150 |
| |
| 摘 要： | 目的采用高效液相色谱法测定双黄连颗粒中绿原酸的含量。方法用外标一点法,Diamonsil ODS1 C18色谱柱,甲醇-水-冰醋酸(15:85:1)为流动相,流速为1.0mL/min,λ=324nm。结果绿原酸在0.060～1.210μg范围内呈良好线性,回归方程为Y=105427X+586.43,r2=0.9995,平均加样回收率为99.3%,RSD=0.82%(n=6)。结论本方法简便、准确,专属性强,测定结果重复性好,为双黄连颗粒中绿原酸的定量分析提供了科学有效的方法 。
|
| 关 键 词： | 双黄连颗粒 绿原酸 高效液相色谱法 含量测定 |
Determination of Chlorogenic Acid in Shuanghuanglian Keli by HPLC |
| |
| ZHANG Hong-tao,CAI Jun-an,GUO Xin-hui. Determination of Chlorogenic Acid in Shuanghuanglian Keli by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2010, 17(4): 43-44 |
| |
| Authors: | ZHANG Hong-tao CAI Jun-an GUO Xin-hui |
| |
| Affiliation: | (Henan Bainian Kangxin Pharmaceutical Co. Ltd, Dancheng 477150, China) |
| |
| Abstract: | Objective To establish the method for detemining the content of chlorogenic acid in Shuanghuanglian Keli by HPLC. Methods Using Diamonsil OD81 C18 column, with methanol-water-glacial acetic acid （15 ： 85 ： 1） as the mobile phase, detection wavelength as 324 nm and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.060-1.210 μg for chlorogenic acid and the equation was Y= 105427X＋586.43, r2=0.999 5. The average recovery was 99.3% and RSD was 0.82% （n =6）. Conclusion This method was simple, accurate and proper, and the reduplication of the result was good, which provide scientific quantitative analysis method of chlorogenic acid in Shuanghuanglian Keli. |
| |
| Keywords: | Shuanghuanglian Keli chlorogenic acid HPLC content determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
