PTEN基因对人恶性黑色素瘤细胞克隆株VEGF的影响

目的：研究肿瘤抑制基因-PTEN诱导表达后对人恶性黑色素瘤（malignant melanoma，MM）细胞株SK-MEL-31VEGF变化的影响，为进一步研究该基因在MM细胞信号传导中的地位和分子生物学作用打下基础。方法：应用分子生物学、免疫学和病理学等技术，研究PTEN基因、反义PTEN基因转染人MM细胞株SK-MEL-31前后细胞内血管内皮生长因子(Vascular end othelial growth factor，VEGF)VEGF的表达情况。结果：发现PTEN基因转染人MM细胞株SK-MEL-31经诱导表达后细胞内VEGF表达明显减少，而转染反义PTEN基因的细胞，其VEGF表达无明显变化。结论：PTEN基因的表达影响MM细胞侵袭和转移等肿瘤病理生物学行为，与细胞内VEGF的变化相关。

THE INFLUENCE OF PTEN TUMOR SUPPRESSOR GENE ON EXPRESSION OF VEGF IN HUMAN MALIGNANT MELANOMA

Objective:To study the influence of PTEN tumor s uppressor gene on expression of VEGF in Malignant Melanoma (MM)cell line,and to further understand the mechanism of action of the gene on the malignant melanoma cell line and make a groundwork for ex-ploiting its localization and molec ular biological function in the sign al transduction pathway of the tumor cells in the future.Methods :We study the influence of PTEN tumor suppressor gene on expression of VEGF in Malignant Melanoma cell line was investigated by using molecular biological,immunologic al and pathological techniques.Results:PTEN significantly lowered VEGF exp ression in the cells tramsfected with PTEN.Conclusion:PTEN gene is a clear related suppressor gene of MM.It plays an important ro le in the mechanisms of the oncogenesis and tumor progression of MM.It is suggested that VEGF p articipates in the mechanism.