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| 流产模型孕鼠脾脏调节性T细胞分离纯化方法的初步建立 | |
| 引用本文： | 殷治华,赵富玺,穆雅琴,刘润花,王健,冯婷婷,齐彦. 流产模型孕鼠脾脏调节性T细胞分离纯化方法的初步建立[J]. 中国实用医药, 2012, 7(6): 5-6 |
| 作者姓名： | 殷治华 赵富玺 穆雅琴 刘润花 王健 冯婷婷 齐彦 |
| 作者单位： | [1]山西医科大学基础医学院,太原030001 [2]大同大学免疫研究所,太原030001 |
| 基金项目： | 国家自然科学基金,国家人口和计划生育委员会立项项目,山西省科学技术发展计划项目 |
| 摘 要： | 目的探索流产模型孕鼠CD4+CD25+T细胞和CD4+CD25-T细胞快速高效的分选方法。方法采用免疫磁珠两步法分离流产模型孕鼠脾脏中T细胞,分离后的细胞经台盼蓝染色检测细胞存活率,经流式细胞仪检测分离的纯度。结果流产模型孕鼠脾脏细胞经过免疫磁珠细胞分选法2次分选得到CD4+/CD25+调节性T细胞纯度为(93.51.1)%,CD4+/CD25-T细胞纯度为(96.60.6)%。结论采用免疫磁珠细胞分选法能够快速高效的得到高纯度的CD4+CD25+T细胞和CD4+CD25-T细胞。 |
| 关 键 词： | 流产模型 调节性T细胞 免疫磁珠细胞分选 流式细胞仪检测 |
The preliminary establishment of the separation and purification methods of regulatory T cells in the spleen of abortion model rat |
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| Affiliation: | YIN Zhi-hua,ZHAO Fu-xi,MU Ya-qing,et al.School of Busic Medical Science of Shanxi Medical University,Taiyuan 030001,China |
| Abstract: | Objective To explore the method of separation of CD4＋CD25＋ T cells and CD4＋ CD25-T cells in abortion model pregnant rat fast and effectively.Methods The immune magnetic beads two-step separation is used to separate T cells of the spleen in abortion model rat,and the cell survival rate was tested by a cell to blue stain after separated,the flow cytometric tests the purity of separation.Results The purity of CD4＋CD25＋regulatory T cells was（93.51.1） % in abortion model pregnant rat by magnetic beads immune cell separation method for tow times,the purity of CD4 ＋CD25-T cells is（96.60.6） %.Conclusion The immune cells magnetic beads separation method of high speed and high efficiency can get high purity of CD4 ＋CD25＋ T cells and CD4 ＋CD25-T cells. |
| Keywords: | Abortion model Regulatory T cells Immune cell sorting magnetic beads Flow cytometric detection |
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