| Application of Serum-Free Culture Medium for Preparation of A-NK Cells |
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| 引用本文： | Zhihua Wang Zhibin Zhang Hui Zhang Yan Zhang. Application of Serum-Free Culture Medium for Preparation of A-NK Cells[J]. Cellular & molecular immunology, 2006, 3(5): 391-395 |
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| 作者姓名： | Zhihua Wang Zhibin Zhang Hui Zhang Yan Zhang |
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| 作者单位： | Institute of Oncology, Harbin Medical University, China |
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| 摘 要： | To compare the differences between proliferation and cytotoxicity of adherent natural killer （A-NK） cells cultured with serum-free medium AIMV and standard serum-containing medium in vitro, and also observe the assisting effect of IL-12 on the activation and the morphology character of IL-2-treated A-NK cells, cellular proliferation was evaluated by MTT method in vitro. The morphology of the target cells killed by A-NK cells was observed through electroscope. All of the A-NK cells cultured in serum-free medium AIMV could rapidly proliferate and keep high cytotoxicity compared with that in standard serum-containing medium. A-NK cells activated by both moderate-dose IL-2 and IL-12 were superior to the high-dose IL-2-treated A-NK cells. These data indicated that serum-free medium AIMV could replace standard serum-containing medium for culturing A-NK cells, and moderate-dose IL-2 and IL-12 could reduce side effects caused by high-dose IL-2. The study provided a new experimental basis for experimental and clinical preparation of A-NK cells. Cellular ＆ Molecular Immunology.
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| 关 键 词： | 血清 介质 A-NK细胞 细胞培养 |
| 收稿时间： | 2006-03-11 |
| 修稿时间： | 2006-10-23 |
Application of Serum-Free Culture Medium for Preparation of A-NK Cells |
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| Zhihua Wang,Zhibin Zhang,Hui Zhang,Yan Zhang. Application of Serum-Free Culture Medium for Preparation of A-NK Cells[J]. Cellular & molecular immunology, 2006, 3(5): 391-395 |
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| Authors: | Zhihua Wang Zhibin Zhang Hui Zhang Yan Zhang |
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| Abstract: | To compare the differences between proliferation and cytotoxicity of adherent natural killer (A-NK) cells cultured with serum-free medium AIMV and standard serum-containing medium in vitro, and also observe the assisting effect of IL-12 on the activation and the morphology character of IL-2-treated A-NK cells, cellular proliferation was evaluated by MTT method in vitro. The morphology of the target cells killed by A-NK cells was observed through electroscope. All of the A-NK cells cultured in serum-free medium AIMV could rapidly proliferate and keep high cytotoxicity compared with that in standard serum-containing medium. A-NK cells activated by both moderate-dose IL-2 and IL-12 were superior to the high-dose IL-2-treated A-NK cells. These data indicated that serum-free medium AIMV could replace standard serum-containing medium for culturing A-NK cells, and moderate-dose IL-2 and IL-12 could reduce side effects caused by high-dose IL-2. The study provided a new experimental basis for experimental and clinical preparation of A-NK cells |
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| Keywords: | A-NK cell serum-free medium AIMV IL-12 |
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