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| 大鼠睾丸白膜下注射12种昆明山海棠单体对其附睾尾精子影响的观察 | |
| 引用本文： | 周激文 骆毅. 大鼠睾丸白膜下注射12种昆明山海棠单体对其附睾尾精子影响的观察[J]. 生殖医学杂志, 1993, 2(3): 165-168 |
| 作者姓名： | 周激文 骆毅 |
| 作者单位： | 昆明云南省计划生育科研所，昆明云南省计划生育科研所，昆明云南省计划生育科研所，昆明云南省计划生育科研所，昆明云南省计划生育科研所，中国科学院昆明植物研究所，中国科学院昆明植物研究所 650021，650021，650021，650021，650021 |
| 基金项目： | 国家计划生育委员会资助 |
| 摘 要： | 本研究通过向Wistar大鼠睾丸白膜下注射从昆明山海棠乙醇提取物(TH_5)中分离而得的12种单体化合物进行抗生育活性初筛。结果表明,其中4种单体7HF、TH_3、TH_(17)和T_8能使大鼠附睾尾精子计数明显下降(P<0.01),最后,并讨论了这种初筛方法的可取与不足之处。 |
| 关 键 词： | 昆明山海棠 大鼠 抗精子发生剂 |
Observation of effect on the epididymal caudal sperms after subaibuginea testis injection of 12 monomer compounds isolated from Tripterygium hypoglaucum(Levl. )Hutch. in rats |
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| ZHOU Jiwen,LUO Yi,PENG Lin,PAN Runeng,LIIU Liwen,ZHANG Xianming,WU Dagang Yunnan Family Planning Institute,Kunming Kuming Institute of Botany,Chinese Academy of Science. Observation of effect on the epididymal caudal sperms after subaibuginea testis injection of 12 monomer compounds isolated from Tripterygium hypoglaucum(Levl. )Hutch. in rats[J]. Journal of Reproductive Medicine, 1993, 2(3): 165-168 | |
| Authors: | ZHOU Jiwen LUO Yi PENG Lin PAN Runeng LIIU Liwen ZHANG Xianming WU Dagang Yunnan Family Planning Institute Kunming Kuming Institute of Botany Chinese Academy of Science |
| Affiliation: | ZHOU Jiwen,LUO Yi,PENG Lin,PAN Runeng,LIIU Liwen,ZHANG Xianming,WU Dagang Yunnan Family Planning Institute,Kunming 650021 Kuming Institute of Botany,Chinese Academy of Science |
| Abstract: | Injection into subalbuginea testes in Wistar rats was used as a screening method for the antifertility activity of 12 monomer isolated from the ethanol extract(TH_5)of Tripterygium hypoglau(?)m(Levl.)Hutch. In control groups,the rats were injected with 7. 5% ethanol 0. ]ml or with gossypol 0. 5 or 1.0mg/7. 5% ethanol 0. 1ml for per testis. In experimental groups,the rats were injected with a simple 1. 0mg/7. 5% ethanol 0. 1ml respectively. Once a week for 2 weeks,on the 15th day brought up for anatomy. The results showed that THF,TH_3,TH_(17) and T_8 compounds significantly reduced the number of sperms in rat epididymal cauda (P<0. 01),furthermore,THF and T_8 also reduced the sperm motility (P<0. 05). The preliminary screening by means of this method bears many advantages,such as less sample and animal used,short experimental cycle,easiness for comparison and repetition. |
| Keywords: | Tripterygium hypoglaucum(Le(?). )Hutch Rat Antispermatogenesis agent |
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