SOD对高原犬体外循环心肌再灌注损伤的保护作用

目的：探讨SOD在高原地区体外循环中的心肌保护作用。方法：将20只杂种犬随机分成A组（对照组，n=10）和B组（试验组n=10)，模拟临床浅低温体外循环，B组停博液中加入SOD6万U/100ml，分别于CPB前、30min,60min及CPB后2h取样测定心肌组织中的MDA，冠状静脉血中CPK浓度以及心肌组织的病理改变，比较两组的差异。结果：A组心肌组织中MDA含量在CPB60min及CPB后2h明显高于B组；A组冠状静脉血中CPK浓度在CPB60min及CPB后2h明显高于B组；CPB后A组心肌发生了更明显的损伤性病理改变。结论：SOD具有减轻高原体外循环心肌再灌注损伤的作用。

Effect of superoxide dismutase (SOD) on lschemia-reperfusion injury of the myocardium in dogs during cardiopulmonary bypass (CPB) at high altitude

Objective:To study the protective effect of SOD on the myocardium during CPB at high altitude. Methods:Twenty hybrid dogs were randomly divided into two groups: control group(group A, n=10) and SOD group (group B, n=10). Dog models of CPB with hypothermia were established, in which serum malmondialdehyde (MDA) level, creatine phosphokrnase (CPK) concentration in coronary venous blood and the pathologic changes of myocardium were respectively measured before CPB, during CPB(at the 30th minute and 60th minute) and two hours after CPB. Results:Comparison between the two groups revealed significant higher MDA levels and CPK concentration in group A during CPB (at the 60th minute) and two hours after CPB, and more serious pathologic changes of myocardium were observed in group A. Conclusion:SOD cnd abate the ischemia-reperfusion injury of myocardium during CPB at high altitude.