一氧化氮合成酶mRNA在正常大鼠肾组织中表达的研究

目的：探讨正常大鼠肾组织一氧化氮合成酶mRNA(NOSmRNA)表达的特点。方法 采用组织细胞原位杂交及图像分析技术,对正常大鼠肾组织中 NOS,NOSey NOSRNA表达的定位及含量进行了检测。同时测定肾组织NOS总活性。结果eNOS,nNOS及iNOS在正常肾组织中均有表达。eNOS和nNOS主要分布于肾小球及血管内皮,其中eNOS表达最丰富,髓质明显多于皮质。iNOS含量较低,且仅分布于皮质远,近曲小管上皮,结论 生理情况下,肾组织cNOS的表达量主要决定于eNOS的表达,其高表达对维持肾脏正常功能具有重要作用。

The expression of nitric oxide synthase mRNA in normal rat kidney

Objective To investigate the expression of nitric oxide synthase (NOS) mRNA in normal rat kidney. Methods The expression and content of three kinds of NOS mRNAs of Wistar rats kidney were detected by in situ hybridization and graphic analysis technique. NOS activity of renal tissues was also detected. Results eNOS,nNOS and iNOS were expressed in normal kidney. Both e NOS and nNOS were mainly localized in glomeruli and blood vessel endothelium; eNOS content was richest and that was in renal medulla more than in renal cortex. However, iNOS content was low relatively , and was in distal and proximal tubules epithelial of renal cortex. Conclusion The expression of eNOS play an important role in maintain normal function of kidney.