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| 川芎嗪对大鼠肾小管细胞钙转运的影响 | |
| 引用本文： | 梁翠茵 程锦轩. 川芎嗪对大鼠肾小管细胞钙转运的影响[J]. 中国药理学与毒理学杂志, 1995, 9(4): 274-276 |
| 作者姓名： | 梁翠茵 程锦轩 |
| 作者单位： | 中国医学科学院基础医学研究所，北京高等医学专科学校药理学教研室 |
| 摘 要： | 用Ｐｅｒｃｏｌｌ密度梯度离心法分离大鼠肾皮质远曲小管细胞（ＤＴＣ）和近曲小管细胞（ＰＴＣ）．应用放射性同位素￣（４５）Ｃａ示踪技术观察川芎嗪对Ｃａ￣（２＋）跨膜转运的影响，并且采用蛋白质竞争结合法检测了胞膜腺苷酸环化酶活性及细胞内环磷酸腺苷（ｃＡＭＰ）含量。结果表明川芎嗪明显抑制ＰＴＣ和ＤＴＣ细胞￣（４５）Ｃａ的摄取，但对￣（４５）Ｃａ的外排无明显影响。川芎嗪对ＰＴＣ和ＯＴＣ胞膜腺苷酸环化酶活性无影响，但使细胞内ｃＡＭＰ含量显著降低。结果提示川芎嗪影响肾小管细胞钙的摄取，其机理可能与其诱导其它物质使细胞内ＣＡＭＰ水平降低有关。 |
| 关 键 词： | 川芎嗪，钙放射性同位素，肾小管，近端，肾小管，远端，上皮，腺苷酸环化酶，腺苷环一磷酸 |
Effect of tetramethylpyrazine on calcium transport of renal tubular cells in rats |
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| LIANG Cui-Yin,CHENG Jin-Xuan,DUAN Jin-Hong,WANG Li, WANG Zhen-Gang. Effect of tetramethylpyrazine on calcium transport of renal tubular cells in rats[J]. Chinese Journal of Pharmacology and Toxicology, 1995, 9(4): 274-276 | |
| Authors: | LIANG Cui-Yin CHENG Jin-Xuan DUAN Jin-Hong WANG Li WANG Zhen-Gang |
| Abstract: | In order to know howtetramethylpyrazine(TMP)acts on renal calcium trans-port,proximal tubular cells(PTC) and distal tubularcells(DTC)were separated by using Percoll's gradientmethod. 45Ca was used to study the influence of TMPon calcium transmembrane. It was found that TMPsignificantly reduced the 45Ca uptake of PTC and DTC,but did not influence the 45Ca effiux. TMP also reducedthe cAMP content in PTC and DTC.but did not influ-ence the activity of adenylcyclase.The results suggestthat TMP takes part in regulating the Ca2 transportof PTC and DTC and its effect may be related to the re-duction of cAMP content in the cells. |
| Keywords: | tetramethylpyrazine calciumradioisotopes kidney tubules proximal kidney tubules distal epithelium adenyl cyclase adenosine cyclicmonophosphateSupported bv the National Natural Science Foundationof China.NO 38970833 |
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