氟斑牙发生与氧化应激的研究

目的探讨氟斑牙发生过程中是否存在氧化应激反应。方法80只SD大鼠按雌雄各半体重均等原则随机分为4组，即饮水氟含量50．00mg／L（H）组、25．00mg／L（M）组、12．50mg／L（L）组和0．08mg／L（C）组。饲养16周后处死大鼠，测血清及肝、肾组织中丙二醛（MDA）含量、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物（GSH—Px）活性。结果H、M组及部分L组大鼠下切牙釉质出现棕白相间条纹等典型氟斑牙症状。随着染氟剂量的增加，H、M、L组大鼠血清、肝MDA含量较C组有升高趋势（P〈0．01或P〈0．05），血清及肝、肾SOD、CAT活性较C组有降低趋势（P〈0．01或P〈0．05），GSH—Px活性变化无统计学意义（P〉0．05）。结论氟斑牙发生过程中大鼠体内可能存在氧化系统与抗氧化系统的失衡，氧化应激反应可能是氟中毒早期症状之一。

Study the relationship between the dental fluorosis and oxidative stress

Objective To study the relationship between the dental fluorosis and oxidative stress. Methods Eighty SD rats were randomly divided into 4 groups, sex in half and weight on equality. The experimental groups drinking water with 50 mg F/L （H） ,25mg/L（M） ,12.5mg/L（L） and the control group with 0.08mg/L （C）. All rats were executed at the end of the 16th week. Then, the contents of malonaldehyde （MDA） , superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GSH- Px） activities in liver and kidney were tested. Results The typical symptom of dental fluorosis, a succession of white and brown stripes was observed in rat lower incisors of the H,M and L group. The MDA level increased（ P 〈 0. 01 or P 〈0.05） ,SOD and CAT activities decreased（ P 〈 0. 01 or P 〈0.05 ） of the H,M and L group as fluoride exposure increased. No statistically differ- ence was found in GSH level （ P 〉 0.05 ）. Conclusion The balance of oxidative system and anti - oxidative system may be bro- ken in the dental fluorosis rat. Oxidative stress may be the early symptom of the fluorosis.