|
|||||
|
|
| 人骨髓间充质干细胞向光感受器样细胞诱导分化的研究 | |
| 引用本文： | 徐国兴,侯泽江,徐巍,郭健,谢茂松,白月. 人骨髓间充质干细胞向光感受器样细胞诱导分化的研究[J]. 国际眼科杂志, 2011, 11(1): 14-18. DOI: 10.3969/j.issn.1672-5123.2011.01.006 |
| 作者姓名： | 徐国兴 侯泽江 徐巍 郭健 谢茂松 白月 |
| 作者单位： | 福建医科大学附属第一医院眼科中心,福建省眼科研究所,中国福建省福州市,350005 |
| 基金项目： | 中国国家自然科学基金重点资助项目(No.60827002); 中国福建省重点科学研究基金资助项目(No.2008Y0040)~~ |
| 摘 要： | 目的:探索骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)定向分化成光感受器样细胞所需的微环境。方法:采用淋巴细胞分离液梯度离心法分离纯化人BMSCs,培养第3代的细胞以碱性成纤维细胞生长因子(bFGF)、表皮生长因子(EGF)及脑源性神经营养因子(BDNF)3种因子首先进行向神经前体细胞诱导分化,应用免疫细胞化学检测巢蛋白及微管相关蛋白-2,当巢蛋白阳性表达率达到最高时更换诱导因子,加入色素上皮衍生因子(PEDF)及牛磺酸(taurine)继续诱导2~3wk,用免疫细胞化学及RT-PCR法检测视紫红质的表达情况。结果:诱导第3d开始能检测到巢蛋白表达,第12d阳性表达率达到最高,达(90.9±2.6)%。微管相关蛋白-2在诱导后第6d检测到阳性表达。部分细胞形态呈神经元细胞样。第12d诱导因子更换为PEDF及taurine继续诱导2~3wk,检测到有视紫红质表达,第2wk阳性率为(20.7±3.8)%,第3wk阳性率为(89.8±3.7)%。结论:采用分阶段诱导法,应用因子bFGF,EGF,BDNF及PEDF,taurine能在体外诱导BMSCs表达光感受器细胞标志物视紫红质。 |
| 关 键 词： | 骨髓间充质干细胞 分化 光感受器细胞 |
Induction of human bone marrow mesenchymal stem cells differentiation into retinal photoreceptor cells in vitro |
|
| Guo-Xing Xu,Ze-Jiang Hou,Wei Xu,Jian Guo,Mao-Song Xie and Yue Bai. Induction of human bone marrow mesenchymal stem cells differentiation into retinal photoreceptor cells in vitro[J]. International Eye Science, 2011, 11(1): 14-18. DOI: 10.3969/j.issn.1672-5123.2011.01.006 | |
| Authors: | Guo-Xing Xu Ze-Jiang Hou Wei Xu Jian Guo Mao-Song Xie Yue Bai |
| Affiliation: | Guo-Xing Xu,Ze-Jiang Hou,Wei Xu,Jian Guo,Mao-Song Xie,Yue BaiOphthalmic Institute of Fujian Province,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,Fujian Province,China |
| Abstract: | AIM:To explore the microenvironment in which human bone marrow mesenchymal stem cells(BMSCs)differentiated into photoreceptor cells under nutrilits induction medium in vitroMETHODS:High-purity BMSCs could be obtained using lymphocyte separation medium by density gradient centrifugation.In the first stage the third passage cells were induced to neural precursor cells with medium including FGF,EGF and BDNF.The expression of the Nestin and MAP-2 were detected by immunocytochemistry.PEDF and taurine instead of ... |
| Keywords: | bone marrow mesenchymal stem cells differentiation photoreceptor cells |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《国际眼科杂志》浏览原始摘要信息 | |
| 点击此处可从《国际眼科杂志》下载免费的PDF全文 | |