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| 槲寄生凝集素蛋白诱导肿瘤细胞凋亡的实验研究 | |
| 引用本文： | 孙华,魏美娟,许均,钱柳,胡朝英,黄秋愉,程伟志,余奇文,张继英,张冬青,陈雪华. 槲寄生凝集素蛋白诱导肿瘤细胞凋亡的实验研究[J]. 中国微循环, 2009, 13(6): 481-484 |
| 作者姓名： | 孙华 魏美娟 许均 钱柳 胡朝英 黄秋愉 程伟志 余奇文 张继英 张冬青 陈雪华 |
| 作者单位： | 1. 上海复旦大学医学院附属妇产科医院,上海,200011 2. 上海交通大学医学院,上海市免疫学研究所 3. 上海交通大学医学院,瑞金医院消化外科研究所 |
| 基金项目： | 国家自然科学基金资助项目，上海市免疫学研究所资助项目 |
| 摘 要： | 目的研究从中药槲寄生中制备的槲寄生凝集素蛋白诱导肿瘤细胞凋亡机制。方法用经典的染色技术：流式细胞术、MTT法分析经分子生物学方法分离纯化的槲寄生凝集素蛋白诱导肿瘤细胞凋亡形态特征，AP峰值和抑制肿瘤细胞增殖的抑制率以研究其诱导肿瘤细胞凋亡机制。结果槲寄生凝集素蛋白具有诱导肿瘤细胞凋亡和显著抑制和阻断肿瘤细胞增殖且以Caspase依赖的方式发挥其生物学作用的。结论槲寄生凝集素蛋白具有直接抗肿瘤作用，在肿瘤免疫学的基础和临床应用研究中具有前景。 |
| 关 键 词： | 槲寄生凝集素蛋白 细胞凋亡 肿瘤免疫 Caspase-3 |
Research on Mechanism of Tumor Apoptosis Induced by a Mistletoe Lectin Protien |
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| SUN Hua,WEI Mei-juan,XU Jun,QIAN Liu,HU Chao-ying,HUANG Qiu-yu,CHENG Wei-zhi,YU Qi-wen,ZHANG Ji-ying,ZHANG Dong-qing,CHEN Xue-hua. Research on Mechanism of Tumor Apoptosis Induced by a Mistletoe Lectin Protien[J]. Journal of Chinese Microcirculation, 2009, 13(6): 481-484 | |
| Authors: | SUN Hua WEI Mei-juan XU Jun QIAN Liu HU Chao-ying HUANG Qiu-yu CHENG Wei-zhi YU Qi-wen ZHANG Ji-ying ZHANG Dong-qing CHEN Xue-hua |
| Affiliation: | SUN Hua, WEI Mei-juan, XU Jun,QIAN Liu, HU Chao-ying, HUANG Qiu-yu, CHENG Wei-zhi, YU Qi-wen, ZHANG Ji-ying, ZHANG Dong-qing, CHEN Xue-hua (Fudan University attached gynecological and obstetrical hospital, Shanghai 200011, P. R. China;2 . Shanghai Jiaotong University,Shanghai Instititute of Immunology Shanghai 200025, P. R. China;3. Department of surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, school of Medicin, Shanghai JiaoTong Univercity, Shanghai 200025, P. R. China) |
| Abstract: | Objective To investigate the mechanism of the tumor apoptosis induced by a mistletoe lectin protiem（MLP）, which was purified from Chinese mistletoe. Methods ML was purified by molecular biological methods, classical dyeing and flow cytometry. MTF was used to analyze the morphological features, AP peaks, proliferation inhibition rates of the tumor apoptosis cell induced by MLP. Results MLP was found that （ 1 ） induced the apoptosis of tumor cells; （2） inhibited and blocked tumor cell proliferations （3） played its biological role through the caspase-3 dependent pathway. Conclusion MLP has a direct antitumor effect, and possesses good prospects in the basic and clinical application research of the tumor immunology. |
| Keywords: | Caspase-3 |
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