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| 肾综合征出血热病毒大鼠单克隆抗体亲和力及抗原结合位点测定 | |
| 引用本文： | 徐海峰,杨为松,崔龙洙,梁喆,白雪帆,米力,刘原. 肾综合征出血热病毒大鼠单克隆抗体亲和力及抗原结合位点测定[J]. 中华实验和临床病毒学杂志, 1995, 0(2) |
| 作者姓名： | 徐海峰 杨为松 崔龙洙 梁喆 白雪帆 米力 刘原 |
| 作者单位： | 西安解放军第四军医大学唐都医院，西安医科大学附属二院内科 |
| 摘 要： | 应用非竞争ＥＬＩＳＡ和ＥＬＩＳＡ相加试验对８株抗肾综合征出血热病毒（ＨＦＲＳＶ）大鼠单克隆抗体（ＭｃＡｂ）进行了抗体亲和力和抗原结合位点的测定。结果提示这些ＭｃＡｂ至少可识别ＨＦＲＳＶ核壳蛋白（ＮＰ）上６种不同抗原决定簇，并且表明ＮＰ上可能存在ＨＦＲＳＶ组、亚组、型或亚型抗原决定簇。ＭｃＡｂ亲和常数测定的高低排序结果为ＫＦ１２＞ＤＥ１１＞Ｘ１０＞ＣＨ１０＞ＩＧ４。 |
| 关 键 词： | 肾变病综合征，出血热病毒，抗体．单克隆，抗体亲合力，酶联免疫吸附测定 |
Measurement of Affinity and Antigenic Sites of Rat Monoclonal Antibodies against Hemorrhagic Fever with Renal Syndrome Virus |
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| Xu Haifeng, Yang Weisong, Cui Longzhu,et al. Measurement of Affinity and Antigenic Sites of Rat Monoclonal Antibodies against Hemorrhagic Fever with Renal Syndrome Virus[J]. Chinese journal of experimental and clinical virology, 1995, 0(2) | |
| Authors: | Xu Haifeng Yang Weisong Cui Longzhu et al |
| Abstract: | Relative affinity of the five rat monoclonal antibodies anti-HFRSV were determined by non-competive ELISA.The results showed that the affinity of the McAbs could be ranked as following KF12>DF11 |
| Keywords: | Hemorrhagic fever with renal syndrome virus Rat Monoclonal antibody Antigenic site Affinity |
| 本文献已被 CNKI 等数据库收录！ | |