维生素D3对人乳腺癌细胞株表皮生长因子受体mRNA表达的影响

目的 探讨维生素Ｄ３对人乳腺癌细胞株细胞增殖等生物学行为的影响。方法 选用两种人类乳腺癌细胞株ＭＣＦ－７及ＺＲ－７５－１，应用细胞培养及核酸分子杂交方法，研究使用维生素Ｄ３以及并用三苯氧胺后，上述细胞株生长及表皮生长因子受体（ＥＧＦＲ）ｍＲＮＡ表达的改变。结果 （１０＾－１０～１０＾－７）ｍｏｌ／Ｌ维生素Ｄ３对ＭＣＦ－７及ＺＲ－７５－１细胞呈现明显的抑制生长作用，并能抑制（１０＾－９～１０＾－７）

Modulating effect of vitamin D3 in vitro on EGFR mRNA expression of human breast cancer cell lines]

OBJECTIVE: To assess the modulating effects of vitamin D3, combined with or without tamoxifen, on cell proliferation and EGFR mRNA level of human breast cancer. METHODS: Two breast cancer cell lines (MCF-7 and ZR-75-1) were treated with various concentrations of vitamin D3, alone or in combination with tamoxifen, for 6 days. Cell proliferation was assessed by MIT uptake and EGFR mRNA level by Northern blot. RESULTS: 10(-10)-10(-7) mol/L vitamin D3 strongly inhibited proliferation of the 2 breast cancer lines, while 10(-9)-10(-7) mol/L tamoxifen stimulated proliferation of MCF-7 cell. The growth inhibitory effect on the 2 cell lines could be augmented and accompanied by a decrease in EGFR mRNA level after combined treatment with IC50 dose of tamoxifen and 10(-10)-10(-9) mol/L vitamin D3. CONCLUSION: Vitamin D3 can be considered as a useful anti-tumor drug in the treatment of breast cancer.