活血化瘀药对骨髓造血的影响

本文用粒系祖细胞(CFU-GM)及经液体培养一周骨髓细胞基质层,同种骨髓粒系祖细胞(称为 SL-CFU-GM),分别作为检测骨髓造血细胞及造血基质功能的方法。观察活血化瘀药对造血的影响。将小鼠给予川芎嗪、复方丹参液,并以环磷酰胺及生理盐水作对照,取骨髓测定 CFU-GM、SL-CFU-GM。结果活血药均不能直接促进造血细胞增殖。但 SL-CFU-GM 明显高于对照组,加消炎痛后 SL-CFU-GM 更形增多。说明活血药促进基质功能,且与 PGE 有关。提示活血化瘀药在体内有改善骨髓造血微环境,从而促进造血。为临床应用活血药于造血系统疾病,提供了实验基础。

Effect of Promoting Blood Circulation to Remove Blood Stasis Herbs on Bone Marrow Hemopoiesis

Our experiment have shown that human or mouse marrow stroma layer(SL)has markedly inhibited the granulocyte-macrophage progenitor cell(CFU-GM)grown over them,called SL-CFU- GM,was taken as a method to investigate the bone marrow hemopoietic cell and the function of hemopoietic stroma.The mechanism of inhibition is,at least partly through prostaglandin(PG)E produced in SL.Mice were treated with promoting blood circulation to remove blood stasis herbs as ligustrazine,a Compound injection of Salvia(composed of Salvia milliorrhizct and Dalbergia odorifera equally),and normal saline(control)for three days.Their bone marrow cells were taken on day 4 for CFU-GM and SL-CFU-GM determination.Both of them did not enhance CFU-GM.But the SL-CFU-GM of mice injected with ligustrazine increased significantly compared to the control(P< 0.05).The promoting effect might be by overcoming,the suppression of PGE of SL,beeause when indomethacin was added,the SL-CFU-GM were further increased.The results suggested that ligustrazine may ameliorate the marrow microenvironment in vivo or the stroma function improved as to enhance hemopoiesis.It may be the reason that some blood disorders with hypoplasia marrow responded well to the PBCRS herbs.