| 成牙本质细胞中上游刺激因子1表达的原位杂交研究 |
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| 引用本文： | 吴礼安,文玲英,杨富生,刘勇. 成牙本质细胞中上游刺激因子1表达的原位杂交研究[J]. 上海口腔医学, 2003, 12(2): 120-122 |
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| 作者姓名： | 吴礼安 文玲英 杨富生 刘勇 |
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| 作者单位： | 第四军医大学口腔医学院,儿童口腔科,陕西,西安,710032;第四军医大学口腔医学院,儿童口腔科,陕西,西安,710032;第四军医大学口腔医学院,儿童口腔科,陕西,西安,710032;第四军医大学口腔医学院,儿童口腔科,陕西,西安,710032 |
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| 摘 要： | 目的 检测、证实成牙本质细胞中上游刺激因子1(USF1)mRNA的表达。方法 用前期制备的T——USF1质粒作模板,借助特异性引物PCR扩增USF1 cDNA片段,回收、随机引物法地高辛标记;培养成牙本质细胞MDPC—23,制备细胞爬片,原位杂交方法检测。结果 成牙本质细胞脑浆中有较强的蓝紫色颗粒。结论 首次证实体外培养的成牙本质细胞中有USF1 mRNA表达,为深入研究USF1分子对成牙本质细胞生长、分化和矿化影响提供了实验依据。
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| 关 键 词： | 成牙本质细胞 上游刺激因子1 mRNA 原位杂交 PCR |
| 文章编号： | 1006-7248(2003)02-0120-03 |
| 修稿时间： | 2003-01-14 |
In situ Hybridization Analysis of USF1 mRNA Expression in Odontoblasts |
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| WU Li-an,WEN Ling-ying,YANG Fu-sheng,LIU Yong.. In situ Hybridization Analysis of USF1 mRNA Expression in Odontoblasts[J]. Shanghai journal of stomatology, 2003, 12(2): 120-122 |
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| Authors: | WU Li-an WEN Ling-ying YANG Fu-sheng LIU Yong. |
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| Affiliation: | Department of Pediatric Dentistry, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China. |
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| Abstract: | Objective To detect and verify USF1mRNA expression in odontoblasts.Methods T-USF1clone prepared previously was used as template and the desired USF1cDNA segment was amplified by PCR with specific primers.The segment was labeled with digoxin as a probe and in situ hybridization was performed on the mounting of odontoblasts.Results Evidently positive staining was shown in the cytoplasm of odontoblasts.Conclusion We verified the expression of USF1mRNA in odontoblasts for the first time and provided evidence for further research. |
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| Keywords: | Odontoblasts USF1 mRNA In-situ hybridization PCR |
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