复方鳖甲软肝片抑制大鼠酒精性肝纤维化作用机制研究

目的探讨复方鳖甲软肝片对实验性大鼠酒精性肝纤维化的抑制作用及机制。方法40只Wistar雄性大鼠随机分为正常组、模型组、中药（复方鳖甲软肝片）治疗组和西药（安珐特）治疗组，以“酒精-吡唑-玉米油”混合液灌胃并饲以高脂饮食16周制备大鼠酒精性肝纤维化模型，给药4周后检测肝纤维化指标、肝组织病理学及肝组织中转化生长因子-β1（TGF—β1）蛋白表达变化。结果酒精性肝纤维化模型大鼠肝功能显著异常，肝纤维化明显；经4周治疗，与模型组比较，中药组及西药组血清中透明质酸（HA）含量显著下降（P〈0．01），且中药组能显著下调大鼠肝组织中TGF—β1蛋白含量。结论复方鳖甲软肝片能够有效阻止和逆转酒精性肝纤维化的进程。

Study on Mechanism of Compound Biejiarangan Troche in Inhibiting Alcohol Hepatic Fibrosis in Rats

Objective To probe into the inhibiting action of Compound Biejiarangan Troche on alcohol hepatic fibrosis in rats. Methods Forty Wistar male rats were divided into control, model, Compound Biejiarangan Troche and Anfate treatment groups. Alcohol hepatic fibrosis model was established by intragastrical perfusion with mixture of ＂alcohol-Pyrazole-coil oil＂ and high-fat diet for sixteen weeks. After four weeks＇ intervention, changes of hepatic fibrosis index, liver tissue pathology and expression of TGF-β1 in liver tissue were observed. Results The hepatic function of model rats were changed and abnormal, with obvious fibrosis in hepatic histopathology. After intervention for four weeks, HA decreased （P〈0.01） in serum of Compound Biejiarangan Troche group and Anfate group compared with that of model group, and expression of TGF-β1 decreased in Compound Biejiarangan Troche group compared with Anfate group. Conclusion Compound Biejiarangan Troche has effect of inhibiting and reversing alcohol hepatic fibrosis in rats.