| 反相高效液相色谱法测定双黄连注射液中咖啡酸的含量 |
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| 引用本文： | 黄京燕,刘占通,雷伟,李幸,吴春丽. 反相高效液相色谱法测定双黄连注射液中咖啡酸的含量[J]. 中国当代医药, 2011, 18(1): 41-42 |
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| 作者姓名： | 黄京燕 刘占通 雷伟 李幸 吴春丽 |
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| 作者单位： | 1. 河南省兽药监察所,河南郑州,450008
2. 郑州大学药学院,河南郑州,450001 |
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| 摘 要： | 目的：建立测定双黄连注射液中咖啡酸含量的高效液相色谱法。方法：采Agilent XDB-C18色谱柱（4.6mm×150mm×5μm）,流动相以甲醇为流动相A,以0.5%磷酸溶液为流动相B,进行梯度洗脱;进样量5μl;流速1ml/min;检测波长为324nm。结果：咖啡酸在进样量在0.03～0.30μg范围内与峰面积线性关系良好（r=0.9997）,平均回收率为99.42%（RSD=1.18%）。结论：方法专属性强、准确、快速,适用于双黄连注射液的质量控制。
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| 关 键 词： | 高效液相色谱法 双黄连注射液 咖啡酸 含量测定 |
Determination of coffeic acid in Shuanghuanglian Injection by RP-HPLC |
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| HUANG Jingyan,LIU Zhantong,LEI Wei,LI Xing,WU Chunli. Determination of coffeic acid in Shuanghuanglian Injection by RP-HPLC[J]. http://www.botanicus.org/, 2011, 18(1): 41-42 |
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| Authors: | HUANG Jingyan LIU Zhantong LEI Wei LI Xing WU Chunli |
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| Affiliation: | 1.Henan Institute of Veterinary Drug Contml,Zhengzhou 450008,China;2.School of Pharmacology of Zhengzhou University,Zhengzhou 450001,China) |
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| Abstract: | Objective：To establish a RP-HPLC method for the determination of coffeic acid in Shuanghuanglian Injection.Methods：The column was Agilent XDB-C18（4.6 mm×150 mm×5 μm）.The mobile phase consisted of methanol-0.5% phosphoric acid with gradient elution;the inject volumes was 5 μl;theflow rate was 1.0 mL/min and the detection wavelength was at 324 nm.Results：The calibration curves were linear within the range of 0.03-0.30 μg（r=0.999 7） for coffeic acid.The average recovery was 99.42%（RSD=1.18%）.Conclusion：The method is rapid and accurate.It can be used for quality control of Shuanghuanglian Injection. |
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| Keywords: | HPLC Shuanghuanglian Injection Coffeic acid Content determination |
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