大鼠皮质神经元的体外培养和纯化

目的：探讨大鼠皮质神经元分离纯化的有效方法。方法：取胎鼠大脑皮质细胞，分别在不同的培养液中进行原代培养，利用相差显微镜对培养的细胞进行动态观察；并以免疫细胞化学技术对神经元进行染色。结果：在相差显微镜下可见加用N2添加剂和胎牛血清培养的神经元生长良好，胞体形态多样，突起数目多，长度长，与邻近神经元的胞体或突起形成接触，而胶质细胞则大量死亡，神经元纯化率达94％以上，未加用N2添加剂培养的神经元胶质细胞大量存在，神经元纯化率达50％左右。结论：N2添加剂和胎牛血清联合应用可使大鼠皮质神经元得到良好的生长和有效纯化。

Culture and Purification of Rat Cortical Neurons in Vitro

Objective\ To study the effective method on rat cortical neurons' purification.\ \{Methods\}\ Rat cortical neurons were cultured with different medium.\ Phase microscopy was used to observe the growth of cells and the neurons were characterized by immunocytochemical technology.\ Results\ The neurons which cultured by N\-2 supplement(a kind of culture medium additive) and fetal bovine serum grew well, the long and abundent processes of neurons were contacted another neurons or its processes.\ Many glial cells were dead.\ The purified rate of neurons was above 94%.\ The purificated rate of neurons which cultured without by N\-2 supplement was only 50%.\ Conclusion\ The use of N\-2 supplement and fetal bovine serum could make neurons grow well and purified effectively.