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| 羟基红花黄色素A对肿瘤上清诱导的人脐静脉内皮细胞增殖的影响 | |
| 引用本文： | 王济,张前,解华,顾立刚,牛旭艳,刘林涛. 羟基红花黄色素A对肿瘤上清诱导的人脐静脉内皮细胞增殖的影响[J]. 中华中医药杂志, 2009, 0(5) |
| 作者姓名： | 王济 张前 解华 顾立刚 牛旭艳 刘林涛 |
| 作者单位： | 北京中医药大学基础医学院; |
| 基金项目： | 国家自然科学基金资助项目(No.30572436) |
| 摘 要： | 目的:研究羟基红花黄色素A(HSYA)对人肝癌细胞株(HepG2)培养上清液诱导的人脐静脉内皮细胞(HUVEC)增殖的抑制作用。方法:原代培养HUVEC,传至第3-6代进行实验。以MTT比色法检测不同浓度HSYA在不同作用时间,对HepG2培养上清液刺激后异常增殖的内皮细胞生长的抑制作用。结果:HSYA在0.0073g/L-0.00045g/L浓度范围内作用24h后,对于肿瘤上清液刺激后异常增殖的HUVEC有显著抑制作用(F=13.015,P<0.01);同样浓度范围作用24h后,对正常HUVEC生长无明显促进或抑制作用。结论:一定浓度的HSYA在一定时间范围内可以显著抑制肿瘤上清液刺激后异常增殖的人脐静脉内皮细胞。提示HSYA有可能成为一种新的肿瘤血管生成阻断剂。 |
| 关 键 词： | 羟基红花黄色素A 肿瘤 人脐静脉内皮细胞 血管生成抑制 MTT |
Effect of Hydroxy Safflor Yellow A on the proliferation of human umbilical vein endothelial cells with the stimulus of tumor cell conditioned medium |
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| WANG Ji,ZHANG Qian,XIE Hua,GU Li-gang,NIU Xu-yan,LIU Lin-tao. Effect of Hydroxy Safflor Yellow A on the proliferation of human umbilical vein endothelial cells with the stimulus of tumor cell conditioned medium[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2009, 0(5) | |
| Authors: | WANG Ji ZHANG Qian XIE Hua GU Li-gang NIU Xu-yan LIU Lin-tao |
| Affiliation: | WANG Ji,ZHANG Qian,XIE Hua,GU Li-gang,NIU Xu-yan,LIU Lin-tao (School of Preclinical Medicine,Beijing University of Chinese Medicine,Beijing 100029,China) |
| Abstract: | Objective:To observe the effect of Hydroxy Safflor Yellow A (HSYA ) on the growth and generation of human umbilical vein endothelial cells (HUVEC) with the stimulus of HepG2 tumor cell conditioned medium. Methods:HUVEC were cultured, and MTT was used to observe the inhibition of HSYA of different concentration and action time to the growth of HUVEC with the stimulus of normal or tumor cell conditioned medium. Results:Remarkable inhibition of the growth of HUVEC with the stimulus of tumor cell conditioned me... |
| Keywords: | Hydroxy Safflor yellow A Tumor Human umbilical vein endothelial cells Angiogenesis inhibitor MTT |
| 本文献已被 CNKI 等数据库收录！ | |