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| 肉苁蓉含药血清诱导骨髓间充质干细胞向成骨细胞分化的实验研究 | |
| 引用本文： | 曾建春,樊粤光,刘建仁,曾意荣,易春智,阎亮. 肉苁蓉含药血清诱导骨髓间充质干细胞向成骨细胞分化的实验研究[J]. 中国骨伤, 2010, 23(8): 606-608. DOI: 10.3969/j.issn.1003-0034.2010.08.015 |
| 作者姓名： | 曾建春 樊粤光 刘建仁 曾意荣 易春智 阎亮 |
| 作者单位： | 1. 广州中医药大学第一附属医院,广东,广州,510405 2. 广州中医药大学 |
| 摘 要： | 目的:研究补肾中药肉苁蓉含药血清对骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)定向分化的影响。方法:全骨髓培养法获得原代骨髓间充质干细胞,经胰酶消化传代培养至第4代,将第4代BMSCs以5×106接种于2块6孔板,每板3组孔,分为空白组、地塞米松组、肉苁蓉组,2 d后换液,加入诱导液。空白组加入含10%胎牛血清(FBS)的L-DMEM培养基继续培养,地塞米松组在基础培养基中加入诱导剂(含β甘油磷酸钠10 mmol/L,地塞米松0.1μmol/L,维生素C 50 mg/L),肉苁蓉组为含10%肉苁蓉含药血清的L-DMEM培养基。第10天取出1块6孔板做ALKP染色,另一6孔板于第20天做6孔板茜素红染色。结果:第10天肉苁蓉组及地塞米松组ALKP染色阳性,第12天可见白色钙结节,第20天茜素红染色阳性。结论:BMSCs在肉苁蓉含药血清诱导下可向成骨细胞分化,分别作为组织工程的种子细胞和诱导因子对治疗骨质疏松、骨折不愈合将有良好的前景。 |
| 关 键 词： | 肉苁蓉 骨髓 间质干细胞 成骨细胞 细胞分化 |
| 收稿时间： | 2009-10-29 |
Experimental study of directional differentiation of bone mesenchymal stem cells(BMSCs) to osteoblasts guided by serum containing cistanche deserticola |
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| ZENG Jian-chun,FAN Yue-guang,LIU Jian-ren,ZENG Yi-rong,YI Chun-zhi and YAN Liang. Experimental study of directional differentiation of bone mesenchymal stem cells(BMSCs) to osteoblasts guided by serum containing cistanche deserticola[J]. China journal of orthopaedics and traumatology, 2010, 23(8): 606-608. DOI: 10.3969/j.issn.1003-0034.2010.08.015 | |
| Authors: | ZENG Jian-chun FAN Yue-guang LIU Jian-ren ZENG Yi-rong YI Chun-zhi YAN Liang |
| Affiliation: | The first Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong,China;The first Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong,China;The first Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong,China;The first Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,Guangdong,China |
| Abstract: | |
| Keywords: | Cistanche deserticola Bone marrow Mesenchymal stem cells Osteoblasts Cell differentiation |
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