| CD137信号对小鼠树突状细胞表面TLR4表达的调节 |
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| 引用本文： | 马倩茹,张烽,周桓,於葛华,张学光,顾宗江. CD137信号对小鼠树突状细胞表面TLR4表达的调节[J]. 苏州大学学报(自然科学版), 2007, 27(1): 11-13,40 |
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| 作者姓名： | 马倩茹 张烽 周桓 於葛华 张学光 顾宗江 |
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| 作者单位： | 苏州大学医学生物技术研究所免疫学教研室,江苏苏州215007 |
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| 基金项目： | 江苏省临床免疫学重点实验室资金资助项目 |
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| 摘 要： | 目的 探讨CD137（4-1BB）激发型单克隆抗体2A对小鼠未成熟树突状细胞株DC2.4表面TLR4表达的调节。方法 以流式细胞术检测不同剂量2A、LPS,或LPS预刺激后再加入2A等因素作用下,DC2.4表面的TLR4表达情况。结果 LPS下调TLR4表达,2A使DC2.4上TLR4表达上调,并拮抗LPS对TLR4的下调作用。LPS预处理后再加入2A,也拮抗LPS的下调作用。结论 CD137信号可以上调DC2.4表面TLR4的表达。
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| 关 键 词： | 树突状细胞 |
| 文章编号： | 1673-0399（2007）01-0011-03 |
| 修稿时间： | 2006-04-12 |
Regulation of Toll-Like Receptor 4 Expression on Murine Dendritic Cells Mediated by CD137 Signal |
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| MA Qian-ru,ZHANG Feng,ZHOU Huan,YU Ge-hua,ZHANG Xue-guang,GU Zong-jiang. Regulation of Toll-Like Receptor 4 Expression on Murine Dendritic Cells Mediated by CD137 Signal[J]. Suzhou University Journal of Medical Science, 2007, 27(1): 11-13,40 |
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| Authors: | MA Qian-ru ZHANG Feng ZHOU Huan YU Ge-hua ZHANG Xue-guang GU Zong-jiang |
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| Affiliation: | Dept of Immunology, Institute of Medical Biotechnology, Suzhou University, Jiangsu Suzhou 215007, China |
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| Abstract: | Objective To study the regulatory effect of agonist CD137(4-1BB)monoclonal antibody 2A on TLR4 expression in a murine immature dendritic cell line DC2.4.Methods Flow cytometry was used to investigate the TLR4 expression on DC2.4 under following conditions:different doses of 2A,LPS or addition of 2A after prestimulation of DC2.4 by LPS.Results LPS could down-regulate TLR4 expression on DC2.4.On the contrary,2A could up-regulate TLR4 expression on DC2.4.Moreover,2A could inhibit the down-regulatory effect of LPS on TLR4 expression.When DC2.4 was stimulated by 2A after LPS prestimulation,2A could still up-regulate TLR4 expression.Conclusion CD137 signal could up-regulate the expression of TLR4 on dendritic cell line DC2.4. |
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| Keywords: | TLR4 4-1BB DC2.4 |
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