庚型肝炎病毒感染中国恒河猴血清学和肝组织学的动 …

目的 探讨庚型肝炎（ＨＧ）发生，发展规律及庚型肝炎病毒（ＨＧＶ）的致病机理。方法 用１例临床和病理确诊的单独ＨＧＶ ＲＮＡ阳性的慢性肝炎患者血清１次或多次分别静脉攻击中国恒河猴，观察攻击前，后动物血清学和肝组织学的动态变化，同时用ＨＧＶ ＮＳ５区抗原制备的单克隆抗体进行肝脏组织免疫组化检测，并于感染后第１８个月体免疫１只雄性猴，取各种脏器观察组织学结构。结果 动物感染后第３０天血清ＡＬＴ，ＡＳＴ开

Dynamic observation of serological and histological changes of Chinese Rhesus monkeys infected by hepatitis G virus

Objective To study the genesis, development and pathogenesis of hepatitis G.Methods The Chinese Rhesus monkeys were intravenously injected with the serum from a hepatitis G patient only with HGV RNA positive. The dynamic changes of serum and liver tissues of the animals were observed before and after infection. Also the immunohistochemical study were done with monocolonal antibody against NS5 gene antigen of HGV. One male monkey was dissected and the internal organs were taken for histological examination 18 months after infection.Results Serum ALT and AST became increasing 30 days after infection and showed abnormal continously for 9 months. Spotty and focal necrosis and piecemeal necrosis were found in liver tissue from 2 to 18 months after infection. Positive staining of HGV antigen was present in cytoplasm of liver cells by immunohistochemistry. Histological observation showed that the internal organs were normal except the liver tissue in which the damages were similar with that in hepatitis. Conclusion The successful infection of Chinese Rhesus monkey by serum from hepatitis G patient can used as a good animal model for the study of hepatitis G.