| Effects of Nephritis No.3 Recipe on Nitric Oxide,Nitric Oxide Synthase Secreted by Cultured Mesangial Cells in Rats and the Gene Expression of Inducible Nitric Oxide Synthase |
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| 作者姓名： | Chen Zhi-qiang Huang Huai-peng Huang Wen-zheng Zhu Xiao-di Lin Qing-qi |
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| 作者单位： | Institute of Integrative Chinese and Western Medicine，Hebei Medical University，Shijiazhuang 050017，Nanjing University of TCM，The Affiliated Hospital of Tianjin College of TCM，The Affilated Hospital of Tianjin College of TCM，The Affiliated Hospital of Tianjin College of TCM |
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| 基金项目： | The item was supported by Tianjin Scientific Committee (No. 953101311)，won the second grade Science and Technology Progress award of Tianjin in 2001 |
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| 摘 要： | Objective:To explore the effect of the Nephritis No.3(N-3)recipe on nitric oxide(NO),nitric oxide synthase(NOS)secreted by cultured mesangial cells(MC)and its gene expression of the in-ducible nitric oxide synthase(iNOS).Methods:The drug(nephritis No.3)-containing serum was preparedwith serum pharmacological technique,and then was applied to react on mesangial cells cultured In fetalcalf serum(FCS)and cells cultured in FCS plus lipopolysaccharide.To observe the secretion of NO andNOS and the gene expression of iNOS by means of RT-PCR.Results:Under the two kinds of culture con-ditions,the content of NO and NOS in the groups with drug-containing serum were higher than thosewithout drug-containing serum(P<0.05,P<0.01),and the expression of iNOS mRNA was up-regula-ted too.Conclusion:The N-3 could significantly promote the secretion of NO and NOS and the mRNA ex-pression of iNOS in rats.
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| 收稿时间： | 13 June 2003 |
Effects of Nephritis No. 3 recipe on nitric oxide, nitric oxide synthase secreted by cultured mesangial cells in rats and the gene expression of inducible nitric oxide synthase |
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| Authors: | Chen Zhi-qiang Huang Huai-peng Huang Wen-zheng Zhu Xiao-di and Lin Qing-qi |
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| Abstract: | Objective:To explore the effect of the Nephritis No. 3 (N-3) recipe on nitric oxide (NO), nitric oxide synthase (NOS) secreted by cultured mesangial cells (MC) and its gene expression of the in-ducible nitric oxide synthase (iNOS). Methods:The drug (nephritis No. 3)-containing serum was prepared with serum pharmacological technique, and then was applied to react on mesangial cells cultured in fetal calf serum (FCS) and cells cultured in FCS plus lipopolysaccharide. To observe the secretion of NO and NOS and the gene expression of iNOS by means of RT-PCR. Results:Under the two kinds of culture conditions, the content of NO and NOS in the groups with drug-containing serum were higher than those without drug-containing serum (P<0. 05, P<0. 01), and the expression of iNOS mRNA was up-regulated too. Conclusion: The N-3 could significantly promote the secretion of NO and NOS and the mRNA expression of iNOS in rats. |
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| Keywords: | Nephritis No 3 recipe mesangial cell nitric oxide nitric oxide synthase |
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