海金沙中非法添加柠檬黄的快速鉴别研究

目的 建立快速鉴别海金沙中非法添加柠檬黄的方法.方法 采用水试法进行柠檬黄初筛;采用薄层色谱法对柠檬黄进行定性鉴别;采用高效液相色谱法进行验证试验,色谱柱为Agilent Eclipse XDB-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.05 mol/L乙酸铵溶液(梯度洗脱),流速为1.0 mL...

Rapid Identification of Lemon Yellow Illegally Added in Lygodii Spora

Objective To establish a rapid method for the identification of lemon yellow illegally added in Lygodii Spora.Methods Aqueous solution test was used for lemon yellow preliminary screening.The thin-layer chromatography(TLC)was used for qualitative identification of lemon yellow,and high-performance liquid chromatography(HPLC)was used for the validation test,the chromatographic column was Agilent Eclipse XDB-C18 column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.05 mol/L ammonium acetate solution(gradient elution),the flow rate was 1.0 m L/min,the detection wavelength was 432 nm,the column temperature was 35℃,and the injection volume was 10μL.Results The test solution of sampleⅤandⅥshowed similar and significant yellow color to the control solution of lemon yellow,the TLC chromatograms showed the same yellow spots on the corresponding position of the reference solution of lemon yellow.The retention time of HPLC chromatograms and the corresponding spectrograms of the test solution of sampleⅤandⅥwere the same as those of the reference solution of lemon yellow,and the negative control had no interference.Conclusion The established aqueous solution test,TLC method and HPLC method are simple,rapid and accurate,which are suitable for rapid screening of lemon yellow illegally added in Lygodii Spora.It is recommended to apply for the supplementary inspection method of lemon yellow in Lygodii Spora.