Effects of age and caloric restriction on cell proliferation in hepatocyte cultures from control and hepatectomized Fischer 344 rats |
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| Authors: | Shaddock, J.G. Chou, M.W. Casciano, D.A. |
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| Affiliation: | 1Divisions of Genetic Toxicology, National Center for Toxicological Research Jefferson, AR 72079 2Divisions of Nutritional Toxicology, National Center for Toxicological Research Jefferson, AR 72079 3University of Arkansa for Medical Sciences, Departments of Biochemistry and Molecular Biology and Toxicology Little Rock, AR 72205, USA |
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| Abstract: | The effects of age and caloric restriction on cell proliferation,measured as scheduled DNA synthesis (SDS), were evaluated inprimary hepatocyte cultures from control and partially hepatectomized(PH) young to old ad libitum (AL) and caloric-restricted (CR)male Fischer 344 (F344) rats. We reported significant age- orCR-related decreases in SDS in control cultures. PH-inducedcultures exhibited significant increases in SDS compared withtheir control counterparts. Hepatocytes from PH-induced oldCR diet-fed animals exhibited significant increases in SDS comparedwith cultures from control old CR, PH-induced young CR and PH-inducedold AL animals. Alternatively, SDS rates for PH-induced youngCR animals were significantly lower at 48 h and higher at 72h than the rates we reported for cultures from PH-induced youngAL F344 rats. These data suggest that CR decreases and preservesthe proliferative capacity in hepatocytes from young animalsand may permit animals to respond more efficiently with inducedcompensatory cellular replication in old age. 4To whom correspondence should be addressed at: National Center for Toxicological Research, Division of Genetic Toxicology, HFT-120, 3900 NCTR Drive, Jefferson, AR 72079, USA |
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