贯叶金丝桃滴丸的提取工艺优选

目的:优选贯叶金丝桃滴丸的提取工艺,为该制剂的剂型改制提供参考。方法:利用药效学试验比较贯叶金丝桃滴丸水提物和醇提物对小鼠感染病毒肺指数的影响,筛选提取方式。以金丝桃苷、绵马贯众素及黄芩苷提取量的综合评分为指标,采用正交试验考察乙醇体积分数、溶剂用量、提取时间及提取次数对贯叶金丝桃滴丸提取工艺的影响。采用HPLC测定金丝桃苷、绵马贯众素及黄芩苷含量,流动相乙腈-0.2%磷酸水溶液(15∶85),检测波长280 nm。结果:最佳提取工艺为加8倍量70%乙醇回流提取3次,每次1.5 h。金丝桃苷、绵马贯众素及黄芩苷提取量分别为1.50,74.57,167.23 mg·g-1。结论:优选的提取工艺稳定可行,适用于贯叶金丝桃滴丸的工业化生产。

Optimization of Extraction Process of Hyperici Perforati Herba Dropping Pills

Objective: To optimize extraction process of Hyperici Perforati Herba dropping pills. Method: Effects of aqueous extract and alcohol extract of Hyperici Perforati Herba dropping pills on lung index of virus infection in mice were compared by pharmacodynamic trials,which was in order to select extraction method.Taking composite score of extracting amounts of hyperoside,dryocrassin and baicalin as index,effects of ethanol concentration,solvent volume,extracting times and time on extraction process were investigated by orthogonal design.HPLC was employed to determine contents of hyperoside,dryocrassin and baicalin with mobile phase of acetonitrile-0.2% phosphoric acid solution(15: 85) and detection wavelength of 280 nm. Result: Optimum extraction process was refluxing extracted 3 times with 8 times the amont of 70% ethanol for 1.5 h each time.Extracting amounts of hyperoside,dryocrassin and baicalin were 1.50,74.57,167.23 mg · g^-1,respectively. Conclusion: This optimized extraction technology is stable,feasible and suitable for industrial production of Hyperici Perforati Herba dropping pills.