远志属7种药用植物ITS1和ITS2序列分析

目的采用ITS序列进行远志属7种药用植物的分子鉴定。方法通过测定远志Polygala tenuifolia、卵叶远志P.sibirica、瓜子金P.japonica、华南远志P.glomerata、蓼叶远志P.persicariifolia、肾果小扁豆P.furcata和小花远志P.arvensis的ITS1、ITS2序列,借助Clustal X、MEGA 3.1软件比较和分析ITS1、ITS2的序列特征。结果远志属7种药用植物ITS1长度为279~291 bp,ITS2的长度为211~219 bp,变异位点232个,信息位点53个;远志与卵叶远志的遗传距离最小,华南远志与肾果小扁豆遗传距离最大,亲缘关系最远。结论远志属7种药用植物的ITS1、ITS2序列可作为分子鉴定的依据。

ITS1 and ITS2 sequences analysis of seven medicinal plants in Polygala L.

Objective To provide the evidence for the molecular identification of seven medicinal plants in Polygala L. Methods Sequencing of ITS1 and ITS2 genes from Polygala furcata, P. japonica, P. tenuifolia, P. glomerata, P. persicariifolia, P. sibirica, and P. arvensis was performed. Sequence features were compared and analyzed using ClustalX and MEGA 3.1 softwares. Results In the seven medicinal plants of Polygala L., the length of ITS1 and ITS2 varied in 279-291 bp and 211-219 bp separately, with 232 variable sites and 53 parsimony information sites. The smallest genetic distance was observed between P. tenuifolia and P. sibirica, and the largest existed between P. furcata and P. glomerata revealing their farest genetic relationship. Conclusion ITS sequences of the seven medicinal plants in Polygala L. can be used as the reference for the molecular identification.