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| HPLC法测定丹栀逍遥片中栀子苷的含量 | |
| 引用本文： | 庄伟霞,陈东鸿,黄晓姗,邹义栩. HPLC法测定丹栀逍遥片中栀子苷的含量[J]. 海峡药学, 2014, 0(8): 36-38 |
| 作者姓名： | 庄伟霞 陈东鸿 黄晓姗 邹义栩 |
| 作者单位： | 庄伟霞 (福建医科大学附属第一医院福州350001)； 陈东鸿 (福建省药品检验所福州350001)； 黄晓姗 (福建省药品检验所福州350001)； 邹义栩 (福建省药品检验所福州350001)； |
| 摘 要： | 目的建立HPLC法测定丹栀逍遥片中栀子苷的方法。方法色谱柱：SYNCRONIS AQ（4.6mm×250mm,5μm）,流动相为乙腈-水（15∶85,v/v）,柱温为室温,流速：1.0mL·min-1,检测波长为238nm。结果栀子苷对照品在0.117-0.780μg的范围内与峰面积积分值呈良好的线性关系（R=0.9998）;平均加样回收率为99.84%,RSD为：0.82%（n=6）。结论本法灵敏、准确、重现性好,可用于丹栀逍遥片的质量控制。 |
| 关 键 词： | 高效液相色谱法 丹栀逍遥片 栀子苷 含量测定 |
Determination of Geniposide in Danzhixiaoyao Tablets by HPLC |
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| Affiliation: | ZHUANG Wei-xia ,CHEN Dong-hong,HUANG Xiao-shan,ZOU Yi-xu(1. The First Affiliated Hospital of Fujian Medical University, Fuzhou 350001, China; 2. Fujian Institute for Food and Drug Control, Fuzhou 350001, China) |
| Abstract: | OBJECTIVE To establish a HPLC method for the determination of Geniposide in Danzhixiaoyao Tablets. METHODS The determination was performed by HPLC using a SYNCRONIS AQ（ 4. 6mm × 250 mm,5μm） column at room temperature. The mobile phase consisted of acetonitrile-water（ 15 ∶ 85,v /v） at a flow rate of1. 0mL·min- 1. The UV detection was set at 283 nm. RESULTS The calibration curve of Geniposide was in good linearity over the range of 0. 117 - 0. 780μg（ R = 0. 9998） and the average recovery was 99. 84%（ RSD = 0. 82%,n= 6）. CONCLUSION The method is sensitive,accurate and reproducible,and it could be used for the quality control of Danzhixiaoyao Tablets. |
| Keywords: | HPLC Danzhixiaoyao Tablets Geniposide Determination |
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