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| 高效液相色谱法测定复方胆通胶囊中穿心莲内酯和脱水穿心莲内酯的总含量 | |
| 引用本文： | 谢浙裕,柴军. 高效液相色谱法测定复方胆通胶囊中穿心莲内酯和脱水穿心莲内酯的总含量[J]. 海峡药学, 2014, 0(7): 71-73 |
| 作者姓名： | 谢浙裕 柴军 |
| 作者单位： | 谢浙裕 (浙江省绍兴市食品药品检验所绍兴312071)； 柴军 (浙江省绍兴市食品药品检验所绍兴312071)； |
| 摘 要： | 目的建立测定复方胆通胶囊中穿心莲内酯和脱水穿心莲内酯的总含量的高效液相色谱法。方法采用高效液相色谱法，AgilentZor-baxExtend—C18色谱柱（4．6mm×250mm，5μm）；流动相：甲醇·水梯度洗脱；流速：1．0mL·min^-1；紫外检测波长：230am、250nm。结果穿心莲内酯和脱水穿心莲内酯线性范围分别为0．9890～9．890μg·mL^-1、1．0560～10．560μg·mL-1范围内呈良好的线性关系（均为r=0．9999），平均回收率分别为98．62％、98．89％（n=9），RSD=2．0％、RSD=1．8％。结论本方法操作简便。结果准确、可靠。 |
| 关 键 词： | 穿心莲内酯 脱水穿心莲内酯 复方胆通胶囊 HPLC |
Determination of Andrographolide and Dehydroandrographolide in Compound Dantong Capsules |
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| XIE Zhe-yu,CHAI Jun. Determination of Andrographolide and Dehydroandrographolide in Compound Dantong Capsules[J]. Strait Pharmaceutical Journal, 2014, 0(7): 71-73 | |
| Authors: | XIE Zhe-yu CHAI Jun |
| Affiliation: | ( Zhejiang Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China) |
| Abstract: | OBJECTIVE To establish a method for the determination of Andrographolide and Dehydroandrographolide in Compound Dantong capsules by HPLC. MEHTODS A Zobax Extend-C18 column was used. The mobile phase was Methanol-Waters with gradient elution analysis and the detection wavelength was 230nm and 250nm. RESULTS The linear range for Andrographolide and Dehydroandrographolide were 0. 9890 - 9. 890μg · mL^-1,1. 0560 - 10. 560μg（ r = 0. 9999 ）, the average recovery were 98.62% ,98.89% （n = 9）, RSD = 2. 0%, RSD = 1. 8%. CONCLUSION The method used for the determination of Andrographolide and Dehydroandrographolide in Compound Dantong capsules is simple,timesaving and accurate. |
| Keywords: | Andrographolide Dehydroandrographolide Compound Dantong capsules HPLC |
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