Tauroursodeoxycholate ameliorates reperfusion injury after pig liver transplantation |
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| Authors: | M. Hertl M. Catherine Hertl Philip Kunkel Silke Schilling Bertrand Prevot Dietrich Kluth Massimo Malagó Christoph E. Broelsch |
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| Affiliation: | (1) Department of Pediatric Surgery, University Hospital Eppendorf, Hamburg, Germany, DE;(2) Department of General and Transplantation Surgery, University Hospital Essen, Hufelandstrasse 55, D-45 122 Essen, Germany Fax: + 49–201–840–55–74, DE |
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| Abstract: | Reperfusion injury is a serious problem after clinical liver transplantation, often leading to dys- or even non-function of grafts. The present study was designed to determine whether the hydrophilic bile salt tauroursodeoxycholate (TUDC), known to be hepatoprotective in cholestatic liver disease, mitigates reperfusion injury in an in vivo pig liver transplantation model. Liver transplantation was performed in 12 pigs after a preservation time of 8 h. TUDC was administered to donor and recipient animals, and saline to controls. Blood was drawn at different time points for determination of liver enzymes. Bile samples were collected, and bile flow (BF), and bile salt secretion rate (BSSR) determined. Samples of liver tissue and bile ducts were taken for assessment by light and electron microscopy. Liver enzymes were significantly lower in the TUDC group. BF and BSSR were significantly higher. Microscopy revealed better preservation of bile duct architecture of the TUDC-infused animals. We can conclude that infusions of TUDC in pig livers ameliorate reperfusion injury in vivo. The molecular basis for this finding may be the membrane stabilizing effect of TUDC. Further studies are warranted to clarify its effect. Received: 19 February 1999 Received after revision: 23 August 1999 Accepted: 16 September 1999 |
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| Keywords: | Pig Liver transplantation Tauroursodeoxycholate Reperfusion injury Liver enzymes |
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