提高心肌梗塞-心衰大鼠模型动物存活率的实验研究

如何提高心肌梗塞- 心衰大鼠的动物存活率和模型成功率,是涉及冠心病诊断和治疗的医学实验的关键技术之一。对600 只大鼠行冠状动脉结扎,建立心肌梗塞和心衰模型,分批观察其存活率,并经高频超声进行评估和鉴定模型建立的成功率,反馈指导模型的建立。结果显示:随着技术的改进和手法的熟练,借助超声心动图检查新技术协助,分批进行的动物存活率和模型建立的成功率明显提高( 存活率:40 % ,72 % ,P< 0-001) ;心衰模型成功率:(38 % ,55 % ,P< 0-01) 。表明必要的技术熟练和术前、术中和术后的护理,以及超声频探头的协助进行技术性回顾和总结,可以大幅度提高实验动物模型建立的存活率和成功率。100 只动物模型的建立可能是技术稳定和成熟的最少数量

The Experimental Study of Improving the Survival Rat e of Rat in Myocardial Infarction Model

It is one of critical techniques for medical experiment based on myocardial infarction-heart failure rat model to improve survival rate of animals. The model of myocardial infarction-heart failure was made through ligating the anterior-descending coronary artery in 600 rats. The results show that the survival rate and the successful rate for the rats model of heart failure were significantly improved (survival rate from 40% to 72%, P<0.05; successful rate from 38% to 55%, P<0.05) with technical training, caring during the operation and retrospectric analysis of the date obtained using high-frequency ultrasound transducer.