增生性瘢痕和瘢痕疙瘩组织中TGF—β1及Ⅰ,Ⅲ型胶原基因 … |
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引用本文: | 唐世杰,庞素芳.增生性瘢痕和瘢痕疙瘩组织中TGF—β1及Ⅰ,Ⅲ型胶原基因 …[J].中华整形烧伤外科杂志,1999,15(4):283-285. |
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作者姓名: | 唐世杰 庞素芳 |
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作者单位: | [1]汕头大学医学院附属第二医院烧伤整形科 [2]湖南医科大学 |
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摘 要: | 目的 检测增生性瘢痕(H)和瘢痕疙瘩(K)组织中TGF-β1及Ⅰ、Ⅲ型前胶原mRNA的表达,了解其相互关系及意义。方法 斑点杂交分析检测H和K组织中Ⅰ、Ⅲ前胶原及TGF-β1mRBA稳态水平的改变;原位杂交检测TGF-β1mRNA在组织中的空间分布。结果 ①K和H组织中TGF-β1mRNA稳态水平明显高于N组和S组;②K选择性I型前胶原mRNA表达增强,而H组织中Ⅰ、Ⅲ前胶原mRNA表达均增强。
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关 键 词: | 增生性瘢痕 瘢痕疙瘩 基因表达 胶原蛋白 |
Changes in TGF-beta 1 and type I, III procollagen gene expression in keloid and hypertrophic scar] |
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Authors: | S Tang S Pang Y Cao |
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Institution: | Department of Burn and Plastic Surgery, Second Affiliated Hospital of Shantou University Medical College, Shantou 515041. |
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Abstract: | OBJECTIVE: The expression of mRNA for TGF-beta 1, type I, III procollagen in keloid and hypertrophic scar was investigated in order to elucidate the pathogenesis of and the difference between keloid and hypertrophic scar. METHOD: Dot-blot hybridization analysis was used to examine the type I, III procollagen and the expression levels of steady-state mRNA. In situ hybridization allowed direct assessment of the distribution of TGF-beta 1 mRNA in the tissue. RESULTS: 1. Our study indicated that the expression level of mRNA for TGF-beta 1 significantly increased in keloid and hypertrophic scar compared with normal scar and normal skin. 2. In keloid tissues, type I precollagen mRNA expression was selectively increased. However, in hypertrophic scar, type I and III collagen mRNAs expressions were simultaneously increased. CONCLUSION: TGF-beta 1 plays an important role in the pathogenesis of keloid and hypertrophic scarring. It implicates that distinct molecular mechanisms are operative in the development of keloid and hypertrophic scarring. |
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