豚鼠胆汁酸盐输出泵（BSEP）基因克隆及其在胆结石豚鼠肝组织中的表达分析

 目的：克隆并分析豚鼠BSEP基因全长cDNA序列，检测BSEP基因在胆结石豚鼠肝组织中的表达。方法：采用3'、5'RACE方法扩增获得豚鼠肝组织BSEP基因全长cDNA，用生物信息学方法对其进行分析鉴定。实时荧光定量PCR检测BSEP基因在胆结石豚鼠肝组织的表达。结果：豚鼠BSEP基因全长cDNA5579bp，共包含28个外显子，开放性阅读框（ORF）长为3963bp，编码蛋白长1320aa。该基因在胆结石豚鼠肝组织的表达较正常豚鼠显著下调（P<0.01）。结论：豚鼠BSEP基因在胆结石豚鼠肝组织中表达下调可能与结石形成有关。

Cloning of guinea pig bile salt export pump (BSEP) and analysis of its expressions in cholelithiasis guinea pig liver

Objective To clone and analyze the full-length cDNA sequence of guinea pigs bile salt export pump gene(BSEP) and detect its expressions in cholelithiasis guinea pigs liver. Methods The BSEP gene full-length cDNA of guinea pigs was amplified by 3'and 5'RACE.Bioinformatic methods were applied to analyz and identify the sequence. Real-time PCR was used to quantification BSEP gene expressions in Cholelithiasis guinea pig liver.Results The length of the guinea pigs BSEP gene cDNA was 5579 bp, containing altogether 28 extrons and a 3963 bp-length open reading frame(ORF) encoding a 1320- amino -acids protein. BSEP mRNA expressions in cholelithiasis guinea pigs liver were significantly decreasses compared to the normal group（P<0.01）. Conclusions Down-regulation of BSEP mRNA expressions in cholelithiasis guinea pigs liver may be relative to the formation of cholelithiasis.