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| PCNA的反义寡核苷酸抑制血管内皮剥脱后SMC增殖的研究 | |
| 引用本文： | 赵狄 徐式斌. PCNA的反义寡核苷酸抑制血管内皮剥脱后SMC增殖的研究[J]. 中国心血管杂志, 1997, 2(3): 131-134 |
| 作者姓名： | 赵狄 徐式斌 |
| 作者单位： | 北京医科大学人民医院心内科 北京100044(赵狄)，北京医科大学人民医院心内科 北京100044(徐成斌) |
| 摘 要： | 本研究采用增殖细胞核抗原(PCNA)的反义寡核苷酸,探讨其抑制大鼠血管内皮剥脱1周时平滑肌细胞(SMC)的增殖反应(每只大鼠给200μg寡核苷酸).结果发现:PCNA的正义寡核苷酸及Pluronic F127(用于转移寡核苷酸)对大鼠血管内皮剥脱1周时SMC的增殖无显著影响(与内皮剥脱组相比,P>0.05).PCNA的反义寡核苷酸一定程度地抑制大鼠血管内皮剥脱1周时SMC的增殖(与内皮剥脱组相比,P<0.01,与正常组相比,P<0.01.说明PCNA的反义寡核苷酸抑制大鼠血管内皮剥脱后SMC的增殖. |
| 关 键 词： | 球囊损伤 平滑肌细胞 增殖 反义寡核苷酸 |
Antisense proliferation cell nuclear antigen oligodeoxynucleotides inhibit SMC profliferation in vi- vo. |
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| Zhao Di,Xu Chengbin. People's Hospital of Beijing Medical university,Beijing. Antisense proliferation cell nuclear antigen oligodeoxynucleotides inhibit SMC profliferation in vi- vo.[J]. Chinese Journal of Cardiovascular Medicine, 1997, 2(3): 131-134 | |
| Authors: | Zhao Di Xu Chengbin. People's Hospital of Beijing Medical university Beijing |
| Affiliation: | Zhao Di,Xu Chengbin. People's Hospital of Beijing Medical university,Beijing,100044 |
| Abstract: | We research the effect of antisense proliferation cell nuclear antigen (PCNA ) oligodeoxynucleotides (ODN) on SMC proliferaiton in vivo after balloon injury. We use 200 ug phosphorothioate ODN for every rat. The result is as follows: SMC significantly proliferate in artery one week after balloon injury (compared to normal artery, P<0. 0001). Sense PCNA ODN and Pluronic F127 that has been used to transfer ODN don't affect SMC proliferation in rat carotid artery one week after balloon injury (compared to injured artery, P<0. 05). Antisense PCNA ODN can inhibit SMC proliferation in rat carotid artery one week after balloon injury (compared to normal artery, P<0. 01, compared to injured artery, P<0. 01). The conclusion is that antisense PCNA ODN can inhibit SMC proliferation in rat carotid artery one wek after balloon injury. |
| Keywords: | balloon injury SMC proliferation antisense oligodeoxynucleotides |
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