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| 反义细胞周期素D1基因对肝癌细胞的作用 | |
| 引用本文： | 肖震宇,陈孝平,黄志勇. 反义细胞周期素D1基因对肝癌细胞的作用[J]. 华中科技大学学报(医学版), 2005, 34(6): 651-654 |
| 作者姓名： | 肖震宇 陈孝平 黄志勇 |
| 作者单位： | 华中科技大学同济医学院附属同济医院肝脏外科,武汉,430030 |
| 基金项目： | 中国博士后科学基金资助项目(No.2004036439) |
| 摘 要： | 目的研究反义基因封闭技术体外抑制细胞周期素D1（cyclin D1）表达后对肝癌细胞增殖的影响。方法肝癌HepG2细胞被转染表达cyclinD1反义互补脱氧核苷酸（AScDNA）的质粒后，观察其cyclinD1表达及体外增殖活性的改变。结果cyclinD1反义cDNA可特异性地抑制肝癌细胞，cyclinD1蛋白的表达。从而调控细胞周期，抑制肝癌细胞增殖。结论利用cyclin D1反义cDNA进行细胞周期干预对肝癌的治疗具有潜在的意义。 |
| 关 键 词： | 细胞周期蛋白D1 反义脱氧核苷酸 肝癌细胞 |
| 修稿时间： | 2005-06-23 |
Effect of Cyclin D1 Antisense cDNA on Hepatocarcinoma Cells |
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| Xiao Zhenyu,Chen Xiaoping,Huang Zhiyong. Effect of Cyclin D1 Antisense cDNA on Hepatocarcinoma Cells[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2005, 34(6): 651-654 | |
| Authors: | Xiao Zhenyu Chen Xiaoping Huang Zhiyong |
| Affiliation: | Xiao Zhenyu,Chen Xiaoping,Huang ZhiyongCenter of Hepatic Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 |
| Abstract: | Objective To investigate the effect of in vitro antisense cDNA of cyclin D1 on the cyclin D1 gene expression and cell proliferation of human hepatocarcinoma.Methods Plasmids containing cyclin D1 antisense cDNA were constructed and transfected into HepG2 cells. Their effects on cell proliferation were examined by MTT method, RT-PCR method, immunohistochemical study and flow cytometry.Results cyclin D1 antisense cDNA could specifically inhibit the expression of cyclin D1 mRNA and protein and regulate cell cycle and cell proliferation of HepG2 cells.Conclusion This therapeutic potential of cyclin D1 antisense cDNA may lead to new cell-cycle-based antitumor strategies for advanced HCCs. |
| Keywords: | cyclin D1 antisense cDNA hepatocarcinoma cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |