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| 维药西帕依溃结安对大鼠溃疡性结肠炎模型COX-2表达的影响 | |
| 引用本文： | 勾晓玉,库热西·玉努斯,黄静静,黄国虹,杨梅,哈木拉提·吾甫尔. 维药西帕依溃结安对大鼠溃疡性结肠炎模型COX-2表达的影响[J]. 医学争鸣, 2009, 30(6): 501-504 |
| 作者姓名： | 勾晓玉 库热西·玉努斯 黄静静 黄国虹 杨梅 哈木拉提·吾甫尔 |
| 作者单位： | 勾晓玉,库热西·玉努斯,黄静静,黄国虹,杨梅,GOU Xiao-Yu,Kurexi·Yunusi,HUANG Jing-Jing,HUANG Guo-Hong,YANG Mei(新疆医科大学生物化学与分子生物学教研室,新疆,乌鲁木齐,830054);哈木拉提·吾甫尔,Halmurat·Wufer(新疆医科大学新疆地方病分子生物学重点实验室,新疆,乌鲁木齐,830054) |
| 摘 要： | 目的：研究维药西帕依溃结安（KJA）对溃疡性结肠炎模型大鼠结肠组织中环氧化酶2（COX-2）表达的影响．方法：采用2，4-二硝基氯苯与乙酸复合法制备大鼠溃疡性结肠炎模型，分为5组进行实验．应用lit—PCR和WesternBlot方法分别检测各组COX-2的mRNA和蛋白质表达水平．结果：KJA大剂量干预组COX-2的蛋白质表达水平与生理盐水阴性对照组相比均明显下调，差异有统计学意义（P〈0．05）；KJA中剂量干预组和5-氨基水杨酸治疗组的COX-2的mRNA表达水平与生理盐水阴性对照组相比明显下调，差异有统计学意义（P〈0．05）．结论：KJA对溃疡性结肠炎的治疗作用可能与下调cox-2的表达有关，而其调节COX4的表达可能发生在转录水平上． |
| 关 键 词： | 结肠炎 溃疡性 环氧化酶2 维药西帕依溃结安 |
Effect of Xipayi Kuijie'an on COX-2 gene expression in rat ulcerative colitis |
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| GOU Xiao-Yu,HUANG Jing-Jing,HUANG Guo-Hong,YANG Mei. Effect of Xipayi Kuijie'an on COX-2 gene expression in rat ulcerative colitis[J]. Negative, 2009, 30(6): 501-504 | |
| Authors: | GOU Xiao-Yu HUANG Jing-Jing HUANG Guo-Hong YANG Mei |
| Affiliation: | GOU Xiao-Yu1,Kurexi·Yunusi1,HUANG Jing-Jing1,HUANG Guo-Hong1,YANG Mei1,Halmurat·Wufer2 1Department of Biochemistry,College of Xinjiang Medical University,Urumqi 830054,China,2Xinjiang Laboratory of Molecular Biology Endemic Disease |
| Abstract: | AIM:To study the effect of the Uygur medicine Kuijie'an(KJA)on the expression of cyclooxygenase-2(COX-2)in intestinal tissues of rat with ulcerative colitis(UC).METHODS:UC Wistar rats induced by 2,4-dinitrochlorobenzene(DNCB)and acetic acid were divided into 5 groups.The methods of semi-quantitation RT-PCR and Western Blot were used to detect the different expression of mRNAs and proteins in each group.RESULTS:In comparison with the normal saline treated negative control group(NS),the expression levels of C... |
| Keywords: | colitis ulcerative cyclooxygenase-2 Kuijie'an(KJA) |
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