| RT-PCR评估直肠冲洗液的量对直肠癌保肛术中清除肿瘤脱落细胞的影响 |
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| 引用本文： | 梁翙,;蔡清萍,;丁健民,;徐建军. RT-PCR评估直肠冲洗液的量对直肠癌保肛术中清除肿瘤脱落细胞的影响[J]. 现代医学, 2014, 0(7): 796-799 |
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| 作者姓名： | 梁翙, 蔡清萍, 丁健民, 徐建军 |
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| 作者单位： | [1]中国人民解放军第455医院普外科,上海200052; [2]第二军医大学附属长征医院普外二科,上海200003 |
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| 摘 要： | 目的：应用RT-PCR评估直肠冲洗液的量（冲洗量）对直肠癌保肛手术中清除肿瘤脱落细胞的影响。方法：对94例进展期直肠癌患者行保肛手术,留取术中冲洗液,按冲洗量分为A、B、C 3组。A组冲洗量1 000 ml,B组冲洗量1 500 ml,C组冲洗量2 000 ml。RT-PCR检测冲洗液中CEA mRNA的表达水平,同时与细胞学检测的结果做对比。结果：A、B、C 3组患者直肠冲洗液肿瘤脱落细胞的CEA mRNA阳性检出率分别为32.3%（10/31例）、19.4%（6/31例）和6.3%（2/32例）,差异具有统计学意义（P=0.03）。3组细胞学阳性检出率分别为19.4%（6/31例）、9.7%（3/31例）和0。结论：随着直肠冲洗量增加,肿瘤脱落细胞的检出率逐步降低,直肠冲洗量至少应在2 000 ml以上,或者行反复冲洗。RT-PCR具有高灵敏度,是细胞学检测很好的补充。
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| 关 键 词： | 逆转录-聚合酶链反应 直肠癌 直肠冲洗 |
Use of RT-PCR to assess the effect of irrigation volume on the efficiency of rectal washout in sphincter-saving resection for rectal cancer |
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| Affiliation: | LIANG Hui, CAI Qing- ping, DING Jian- min, XU Jian-jun (1. Department of General Surgery, the 455th Hospital of PLA, Shanghai 200052, China;2. Department of General Surgery II ,Affiliated Changzheng Hospital,the Second Military Medical University of PLA, Shanghai 200003, China) |
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| Abstract: | Objective： To assess the effect of irrigation volume on the efficiency on rectal washout using RT-PCR. Methods ： 94 cases of advanced rectal cancer were enrolled and intraoperative rectal washout fluids were collected. They were divided into three gro |
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| Keywords: | RT- PCR rectal cancer rectal washout |
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