肺炎支原体感染及IL-6与哮喘急性发作关系的临床研究

目的：研究肺炎支原体感染及IL-6与哮喘急性发作的关系。方法：选择哮喘急性发作息儿31例，肺炎患儿34例，健康儿童30例。采用①酶联免疫吸附试验（ELISA）法检测血清中MP-IgG、MP-IgM特异性抗体；②聚合酶链式反应（PCR）检测MP；③细胞因子（CK）检测ELISA法检测血清中IL-6。结果：①哮喘急性发作及肺炎患儿血清MP-IgG、MP-IgM检测：哮喘组用PCR法或IgM法检测MP阳性率高于肺炎组，差异有统计学意义（P〈0．05），两组MP-IgG阳性率差异无统计学意义（P〉0．05）。②哮喘及肺炎组患儿血清IL-6高于健康对照组，差异有统计学意义（P〈0．01），而IL-6在哮喘及肺炎组间差异无统计学意义（P〉0．05）。③两组MP及其特异性抗体检测一致性比较，差异无统计学意义（P〉0．05）。结论：MP急性感染可能是哮喘急性发作的病原之一，IL-6与MP感染的发病密切相关，所以治疗的关键是控制和消除肺炎支原体感染。

Clinical investigation on correlation of mycoplasma pneumoniae and IL-6 with acute episoded asthma

Objective To investigate the corelation of mycoplasma pneumoniae (MP) and IL-6 with acute episoded asthma. Method For 31 children with acute episoded asthma, 34 with pneumonia and 30 healthy ones were chosen. ①Serum MP-IgG, MP-IgM were detected with ELISA. ②MP were detected with PCR. ③Serum IL-6 were detected with CK-ELISA. Results ①Detection of MP-IgG, MP-IgM in the serum of acute episoded asthma patients and pneumonia patients: The positive rate of the asthma group was higher than that of the pneumonia group with the method of PCR or IgM, and had significant difference (P<0.05);the positive rate of MP-IgG between the two groups showed no significant difference (P>0.05). ②The serum levels of IL-6 in the asthma group and the pneumonia group were higher than that of the healthy group, and had significant difference (P<0.01),but IL-6 level between the asthma group and the pneumonia group had no significant difference(P>0.05).③MP and concordance of it's special antibody between the two groups had no significant difference (P>0.05). Conclusion Acute infection of MP may be one of the pathogenies of acute episoded asthma,and IL-6 has close correlation with MP infection, so controlling and eliminating MP infection is important.