| 腺病毒载体介导蛋白激酶B基因转染大鼠胰岛的研究 |
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| 引用本文： | 张睿,程颖,刘永锋. 腺病毒载体介导蛋白激酶B基因转染大鼠胰岛的研究[J]. 中华实验外科杂志, 2002, 26(1): 871-872. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.020 |
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| 作者姓名： | 张睿 程颖 刘永锋 |
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| 作者单位： | 中国医科大学附属第一医院器官移植科,沈阳,110001; |
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| 摘 要： | Objective To confirm in vitro gene delivery to rat islets by adenovirus vectors. Methods The adenovirus vector, AdS-Aktl, was constructed following the standard molecular methods. The purified islets were infected with either Ad5-EGFP or Ad5-Akt1 adenovirus the multiplicity of infection (MOI) was 500). The expression of Aktl in Ad5-Akt1 islet transfectants was confirmed by using Western blot. Results Adenovirus vector Ad5-Akt1 was constructed and the the titer of the virus was 5.3 × 109 pfu/ml. The transfection efficiency of Ad5-EGFP was 60% -70%, and Akt1 protein was detected in Ad5-Aktl islet transfectants. Conclusion Adenovirus is efficient to deliver exogenous gene into rat islets in vitro.
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| 关 键 词： | 胰岛 腺病毒载体 蛋白激酶B 基因治疗 |
Transfection of adenovirus vector-mediated ptotein kinase B gene into the rat islets |
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| ZHANG Rui,CHEN Ying,LIU Yong-feng. Transfection of adenovirus vector-mediated ptotein kinase B gene into the rat islets[J]. Chinese Journal of Experimental Surgery, 2002, 26(1): 871-872. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.020 |
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| Authors: | ZHANG Rui CHEN Ying LIU Yong-feng |
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| Abstract: | |
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| Keywords: | IsletAdenovirus vectorProtein kinase BGene therapy |
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