不同疗程阿仑膦酸钠对去卵巢大鼠骨骼的影响

目的　探讨阿仑膦酸钠 (固邦 )不同疗程对去卵巢大鼠所致骨质疏松的骨计量学参数的影响。方法　用 3mon龄♀SD大鼠 ,阿仑膦酸盐 1mg·kg- 1 ·d- 1 ,分别灌胃给药 30d和 90d ,体内双荧光标记。实验终止时处死大鼠 ,取左侧胫骨近心端行骨组织形态计量学分析。结果　去卵巢大鼠骨小梁面积百分率分别减少 2 5 % (30d)和 62 % (90d) ,出现骨吸收大于骨形成的高转换型骨质疏松 ;阿仑膦酸钠 30d和 90d均完全对抗去卵巢大鼠的骨高转换。与OVX组比较 ,阿仑膦酸钠作用 30d骨量增加 45 % ,90d骨量增加2 1 9% ,有效防止去卵巢后骨质疏松。结论　阿仑膦酸钠 30d和 90d均通过抑制骨吸收和骨高转换对去卵巢大鼠有预防骨质疏松的作用 ,而阿仑膦酸钠 90d的作用比 30d的强。

Effects of different-term treatment of alendronate on osteoporosis induced by ovariectomized in rats

AIM To investigate the effects of different period of Alendronate in ovariectomized (OVX) rats. METHODS Forty Three 3 month old Sprague Dawley female rats were randomly devided into six groups: sham operated (Sham group 30 d and group 90 d), ovariectomized controls(OVX group 30 d and group 90 d), ovariectomized+alendronate 1 mg·kg -1 ·d -1 (group 30 d and group 90 d). Double in vivo fluorochrome labeling was made. At the end of experiment, the left tibiae were havested for histomorphometrical evaluations. RESULTS Ovariectomized rats trabecular bone mass were reduced markedly because bone resorption excessed bone formation(% Tb.Ar -25% 30 d and -62% 90 d). Treatment with alendronate increased bone volume by 45% in 30 d and by 219% in 90 d and suppressed bone turnover rate by 25% in 30 d and by 62% in 90 d. CONCLUSIONS Treatment with alendronate for 30 d and 90 d can prevent osteoporosis induced by inhibiting bone resorption and bone turnover. The treatment of 90 d was more potent than that of 30 d in protecting agsinst osteoporosis in ovariectomized rats via supression of bone turnover.