SPE-HPLC法测定人血浆中头孢吡肟的浓度

目的:建立测定人血浆中头孢吡肟浓度的方法.方法:采用固相萃取(SPE)血浆中头孢吡肟.色谱柱为大连依利特Hypersil BDS C18,流动相为甲醇-磷酸盐缓冲液(20:80),柱温为30℃,流速为1 ml·min-1,检测波长为257 nm,进样量20 μl.结果:头孢吡肟检测浓度线性范围为1.0～200 mg·L-1(r＝0.999 9);最低检测限为1 mg·L-1;低、中、高3种浓度头孢吡肟的方法回收率分别为118.0%,97.0%,95.0%,日内RSD分别为1.7%,3.6%,2.1%,日间RSD分别为8.2%,4.9%,2.6%.结论:该方法简便、灵敏度高,适用于测定人血浆中头孢吡肟的含量.

Determination of Cefepime in Human Plasma by SEP-HPLC

Objective: To establish a method to determine cefepime in human plasma by SPE-HPLC.Method: Cefepime in plasma was determined by an SPE-HPLC method.The column was an ELITE Hypersil BDS C18 column.The mobile phase consisted of methanol-phosphate buffer solution(20∶ 80),the column temperature was 30℃,the flow rate was 1.0 ml·min-1,the detection wavelength was 257 nm,and the sample volume was 20 μl.Result: The calibration curve of cefepime was linear within the range of 1.0~200 mg·L-1 with good linearity(r=0.999 9).The lowest detection limit was 1 mg·L-1.The recovery was 118.0%,97.0% and 95.0% with intra-day RSD of 1.7%,3.6% and 2.1% and inter-day RSD of 8.2%,4.9% and 2.6% for low,medium and high concentration,respectively.Conclusion: The method is simple and sensitive,and can be used in the determination of cefepime in human plasma.