| 反义寡核苷酸抑制神经母细胞瘤LA-N-5细胞血管内皮生长因子mRNA表达及其生物效应的研究 |
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| 引用本文： | 于芳,唐锁勤,冯晨,尹华,蔡正菊.反义寡核苷酸抑制神经母细胞瘤LA-N-5细胞血管内皮生长因子mRNA表达及其生物效应的研究[J].中国医学文摘：基础医学,2008(5). |
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| 作者姓名： | 于芳 唐锁勤 冯晨 尹华 蔡正菊 |
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| 作者单位： | 中国人民解放军总医院儿内科 |
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| 摘 要： | 目的研究血管内皮生长因子(VEGF)反义寡核苷酸(ASODN)转染对神经母细胞瘤LA-N-5细胞VEGF mRNA表达的影响以及对肿瘤细胞增殖、分化的影响。方法用LipofectamineTM2000介导的VEGF ASODN和错义寡核苷酸(MSODN)转染LA-N-5细胞,半定量RT-PCR检测各组细胞VEGF165和VEGF121 mRNA转染前后不同时间表达的变化;MTT法测定转染后各组细胞的生长曲线及抑制率。结果半定量RT-PCR检测结果显示,在转染后72 h VEGF165和VEGF121 mRNA的表达:ASODN组为0.346±0.029和0.227±0.036,ASODN+LipofectamineTM2000组为0.275±0.035和0.165±0.017。ASODN组和ASODN+LipofectamineTM2000组均显著抑制VEGF mRNA的表达,ASODN+LipofectamineTM2000组抑制作用较ASODN组更强(P<0.05);转染后ASODN组和ASODN+LipofectamineTM2000组细胞增殖显著受抑,在48 h时抑制率最高,分别为(39.92±2.7...
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| 关 键 词： | 神经母细胞瘤 血管内皮生长因子 反义寡核苷酸 基因治疗 |
A study of inhibition on expression of VEGF by antisense oligonucleotides and its biological effect on LA-N-5 cells |
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| Authors: | YU Fang; TANG Suo-qin; FENG Chen; YIN Hua; CAI Zheng-ju |
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| Institution: | YU Fang; TANG Suo-qin; FENG Chen; YIN Hua; CAI Zheng-ju(Department of Pediatrics; General Hospital of PLA; Beijing 100853; China); |
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| Abstract: | Objective To investigate if VEGF ASODN transfection to LA-N-5 cells inhibits VEGF mRNA expression,thus affects its differentiation and proliferation.Methods LA-N-5 cells were transfected with VEGF ASODN or MSODN alone,ordelivered by LipofectamineTM2000,respectively,VEGF mRNA pre-and post transfection expression was detected by semi-quantitative RT-PCR,MTT was employed for evaluation of differentiation and proliferation of cells in each group.Results ①The semi-quantitative RT-PCR detection showed VEGF165 and... |
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| Keywords: | Neuroblastoma Vascular endothelial growth factor Antisense oligodeoxynucleotide Gene therapy |
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