慢性不可预见性应激和氟西汀治疗对雌鼠类抑郁样和焦虑样行为的影响

目的：研究慢性不可预见性应激（CUS）和氟西汀（Flu）治疗对雌鼠类抑郁样和焦虑样行为的影响。方法：成年雌性SD大鼠随机分为NS组、Flu组、CUS＋NS组和CUS＋Flu组,每组6只。CUS＋NS和CUS＋Flu组每日CUS前30 min分别注射2.5 mL/kg NS或10 mg/kg Flu;NS和Flu组仅给予相同剂量的NS或Flu。最后一次应激注射后第2 d用糖水消耗实验和强迫游泳评估类抑郁行为,开场试验评估类焦虑行为。结果：糖水消耗实验示糖水消耗百分比无组间差异,但CUS＋NS组糖水消耗量明显低于其它3组;强迫游泳示CUS＋NS组的不动时间显著长于其它各组,而NS、Flu和CUS＋Flu组间无统计学意义;开场实验示CUS和CUS＋Flu组中央/总路程低于NS和Flu组（P〈0.05）,CUS＋NS组的中央活动时间少于Flu组,CUS＋Flu组的中央活动时间少于NS和Flu组。结论：CUS大鼠具有类抑郁样行为,同时伴类焦虑样行为,Flu治疗可以逆转类抑郁样行为,但对类焦虑样行为无效;Flu对正常大鼠无情绪改变作用。

Effects of Chronic Unpredictable Stress and Fluoxetine Treatment on Depressivelike and Anxiety-like Behaviors of the Adult Female Rats

Objective： To study the effects of chronic unpredictable stress（CUS） and fluoxetine（ Flu） treatment on depressive-like and anxlety-like behaviors of adult female rats. Methods ： Twenty-four virgin fern.ale rats were assigned evenly to normal sodium（NS） group, fluoxetine（Flu） group, chronic unpredictable stress（CUS） exposed plus NS injection group（CUS＋NS）, and CUS exposed plus Flu treatement group（CUS ＋Flu）. CUS ＋NS group and CUS ＋Flu group received a daily intraperitoneal injection of 2.5 mL/kg NS or 10 mg/kg Flu 30 minutes before CUS. NS group and Flu group only received a daily intraperitoneal injection of 2.5 mL/kg NS or 10 mg/kg Flu. The second day after the last in- jection, the sucrose consumption test and forced swim test were used to test the depressive-like behavior and the open field test was used to test anxiety-like behaviors. Results： There was no difference in sucrose consumption percentage, but the sucrose intake had significant difference among groups, with CUS ＋NS groups consuming less sucrose than all other groups. The immobility time had significant difference in 5-min swim test among groups, with CUS ＋NS group longer than the other three groups, and there was no difference among NS, Flu and CUS ＋flu groups. The central-to-total distance traveled ratio in the CUS group and the CUS ＋Flu group was lower than that in the NS group and Flu group, and the sum time in central part in the CUS ＋NS group was less than that in the Flu group, and in the CUS ＋Flu group was less than that in the NS group and the Flu group. Conclusion： CUS induces depressive-like and anxiety-like behavior, and flu treatment may reverse the depressive-like but not the anxiety-like behavior. Flu is devoid of mood-changing effects in normal rats.