3种新型人肿瘤坏死因子衍生物在大肠杆菌中的表？…

Objective　Studies of novel human tumor necrosis factor-α(hTNF-α) derivatives with increased antitumor activity. Methods　Based on the perviousstudies of hTNF-α structure-functionalrelationship, three mutated hTNF-α genes were generated by PCR. These genes were insertedinto E.coli and confirmed by DNA sequencing and Western blot. The antitumor activitieswere determined by mouse L929 cells. Results　Three genes were highly expressed in E.coli. The in vitrocytotoxicity effects of hTNF-α derivatives on L929 cells were 576, 641, 153 times higher than that of naturalhTNF-α. Conclusion　The antitumor activitiesof hTNF-α derivativesare highly improved. And the derivatives have displayed potential prospect for clinicalapplication.

Expression and activityassay of three kinds of novel human tumornecrosis factor-