艾叶不同组分对小鼠急性毒性实验比较研究

目的比较艾叶不同组分对小鼠急性毒性的影响。方法制备艾叶水提组分、挥发油、醇提组分和全组分,采用经典的急性毒性实验方法进行艾叶不同组分小鼠急性毒性比较研究。结果艾叶水提组分、挥发油半数致死量（LD5）0与95%可信限分别为80.2g·kg^-1·d^-1（77.4～83.4g·kg^-1·d^-1）、1.67mL·kg^-1·d-（11.55～1.80mL·kg^-1·d^-1）。醇提组分最大耐受量（MTD）为75.6g·kg^-1·d^-1,全组分最大给药量（MLD）为24.0g·kg^-1·d^-1,分别相当于临床70kg的成人每公斤体重日用量的588.0倍、186.7倍。主要的急性毒性症状谱为怠动、恶心、抽搐、四肢麻痹、俯卧不动。结论艾叶不同组分对小鼠急性毒性强度为：挥发油〉水提组分〉醇提组分〉全组分,但各组分毒性物质基础、体内毒性过程、毒性作用特点、毒性作用机制尚不完全明确,有待于进一步研究。

Experimental Comparison Study on Mice's Acute Toxicity of Different Composition in Folium Artemisiae Argyi

Objective To compare the acute toxicity of different components in Artemsia argyi on mice.Methods Respectively prepare the water extraction components,volatile oil,alcohol extraction components and all components to compare the acute toxicity of different components in Artemsia argyi on mice in accordance with classical acute toxicity test methods.Results The LD50 and 95% confidence limit of water extraction components and volatile oil in Artemsia argyi are respectively 80.2 g·kg-·1d^-1（77.4～83.4 g·kg-·1d^-1）and 1.67 mL·kg-·1d^-1（1.55～1.80 mL·kg-·1d^-1）;The MTD of alcohol extraction components and the MLD of all components are respectively 75.6 g·kg^-1·d^-1 and 24.0 g·kg^-1·d^-1,which are espectively equal to 588.0 times and 186.7 times of 70kg adult＇s daily dried medicinal herb expenses.The main acute toxicity symptoms and signs are negligent respectively equal to 588.0 times and 186.7 times of 70kg adult＇s daily dried medicinal herb expenses.The main acute toxicity symptoms and signs are negligent action,nausea,convulsions,tetraplegia,prone fixed.Conclusion The acute toxicity of different components in Artemsia argyi on mice is：volatile oil water extraction components alcohol extraction components all components,but the toxic substances,process of toxicity in vivo,toxicity characteristics,and toxicity mechanism are all not yet entirely clear,and they are all to be further studied.